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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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SMAD3

SMAD family member 3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Gene Name: SMAD family member 3
Synonyms: SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3
Target Sequences: NM_005902 NP_005893.1 P84022

Publications (1)

1
Zinc supplementation attenuates ethanol- and acetaldehyde-induced liver stellate cell activation by inhibiting reactive oxygen species (ROS) production and by influencing intracellular signaling. Szuster-Ciesielska A, Plewka K, Daniluk J, Kandefer-Szersze M. Biochemical pharmacology. 2009 78:301-14. (WB) [PubMed:19376089]

☰ Filters
SMAD3 (228)
IHC (140)
IHC-P (43)
WB (205)
Flo (10)
DB (8)
ELISA (86)
ICC (25)
IF (46)
IP (7)
rabbit (209)
mouse (19)
No (228)
Viewing 1-25 of 228 items
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Rabbit Polyclonal to Human SMAD3
Xenopus, Chicken, Zebrafish, Pig, Bovine, Mouse, Rat, Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $485.00
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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Mouse Monoclonal [clone AF9F7] (IgG1,k) to Human SMAD3
Mouse, Rat, Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated
50µl   0.5mg/ml   $425.00
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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Rabbit Polyclonal to Human SMAD3
Mouse, Dog, Bovine, Rat, Pig, Macaque, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µg   0.5mg/ml   $425.00
SMAD3 Antibody - SMAD3-S208 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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Rabbit Polyclonal to Human SMAD3
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
SMAD3 Antibody - Western blot of Phospho-Smad3 (T179) antibody
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Rabbit Polyclonal (IgG) to Human SMAD3
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
SMAD3 Antibody - Immunohistochemical analysis of SMAD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human SMAD3
Chicken, Sheep, Rat, Pig, Mouse, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
SMAD3 Antibody - Immunohistochemical analysis of SMAD3 staining in human liver;human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Mouse Monoclonal to Human SMAD3
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $275.00
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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Mouse Monoclonal [clone 7F3] (IgG1,k) to Human SMAD3
Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $495.00
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Mouse Monoclonal [clone 2C12] (IgG2a,k) to Human SMAD3
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $495.00
SMAD3 Antibody - Immunohistochemical analysis of SMAD3 (pS213) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human SMAD3
Chicken, Rat, Pig, Mouse, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl   1mg/ml   $215.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $325.00
SMAD3 Antibody - Immunohistochemistry of paraffin-embedded mouse skin tissue.
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Rabbit Monoclonal (IgG) to Human SMAD3
ICC, IF, IHC, WB
Unconjugated
50µl   $295.00; 100µl   $385.00; 200µl   $560.00
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Rabbit Monoclonal (IgG) to Human SMAD3
Mouse, Rat, Human
ELISA, Flo, IF, IP, WB
Unconjugated
100µl   $530.00
SMAD3 Antibody - Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Rabbit Polyclonal to Human SMAD3
Chicken, Sheep, Rat, Pig, Mouse, Human, Monkey
ICC, IF, IP, WB
Unconjugated
100µl   1mg/ml   $265.00
SMAD3 Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Smad3 (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.
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Rabbit Polyclonal (IgG) to Human SMAD3
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
SMAD3 Antibody - Immunohistochemical analysis of SMAD3 (pT179) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human SMAD3
Mouse, Dog, Sheep, Rat, Pig, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $265.00
SMAD3 Antibody - SMAD3 antibody P100621_T100-NP_005893-SMAD3 (SMAD family member 3) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human SMAD3
Xenopus, Chicken, Zebrafish, Pig, Rat, Mouse, Dog, Guinea pig, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   0.5mg/ml   $325.00
Select
Rabbit Polyclonal to Human SMAD3
Human
ELISA, WB
Unconjugated
100µg   0.5mg/ml   $275.00
SMAD3 Antibody
Select
Rabbit Polyclonal (IgG) to Human SMAD3
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, ICC, IF, IHC, WB
Biotin Conjugated
200µl   $740.00
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Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, ICC, IF, IHC, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, ICC, IF, IHC, WB
APC Conjugated
200µl   $740.00
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Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, ICC, IF, IHC, WB
HRP Conjugated, Azide-free
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, ICC, IF, IHC, WB
PE Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Human SMAD3
Human
ELISA, Flo, ICC, IF, IHC, WB
AP Conjugated
200µl   $740.00

Viewing 1-25 of 228 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).