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IHCPlus™ SMAD3 Antibody aa100‑150 Polyclonal IHC,WB LS‑B2064

Catalog Number Size Price
LS-B2064-50 50 µg (0.5 mg/ml) $425 
Anti-SMAD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ SMAD3 Antibody aa100‑150 Polyclonal IHC,WB LS‑B2064

Note: This antibody replaces LS-C19414, LS-C89102
Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2064-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-B2064 is an unconjugated rabbit polyclonal antibody to SMAD3 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SMAD3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A portion of amino acids 100-150 of human SMAD3.
Epitope
aa100-150
Specificity
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Usage
Immunohistochemistry: LS-B2064 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2064 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

Validation

Anti-SMAD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-SMAD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SMAD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 9/16/2019