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Catalog Number Size Price
LS-C745331-25 25 µl (0.82 mg/ml) $275 
SMAD1 Antibody - Western blot using the Affinity Purified anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL.

Polyclonal Rabbit anti‑Human SMAD1 Antibody (phospho‑Ser206, WB) LS‑C745331

Polyclonal Rabbit anti‑Human SMAD1 Antibody (phospho‑Ser206, WB) LS‑C745331

Antibody:
SMAD1 Rabbit anti-Human Polyclonal (pSer206) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745331-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SMAD1 Rabbit anti-Human Polyclonal (pSer206) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD1 antibody LS-C745331 is an unconjugated rabbit polyclonal antibody to SMAD1 (pSer206) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.
Epitope
pSer206
Specificity
Reactivity occurs against human SMAD1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human SMAD1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with SMAD1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD1
Q15797 NM_005900 NP_005891.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021