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Catalog Number Size Price
LS-C357612-10 10 µg $318 
LS-C357612-100 100 µg (0.5 mg/ml) $470 
SLC1A2 / EAAT2 / GLT-1 Antibody - Western blot analysis of EAAT2 using anti-EAAT2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EAAT2 at approximately 62KD. The expected band size for EAAT2 is at 62KD.
SLC1A2 / EAAT2 / GLT-1 Antibody - EAAT2 antibody Western blot. All lanes: Anti EAAT2 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: U87 Whole Cell Lysate at 40 ug. Lane 4: SMMC Whole Cell Lysate at 40 ug. Lane 5: PANC Whole Cell Lysate at 40 ug. Lane 6: A549 Whole Cell Lysate at 40 ug. Predicted band size: 62 kD. Observed band size: 62 kD.
SLC1A2 / EAAT2 / GLT-1 Antibody - Western blot analysis of EAAT2 using anti-EAAT2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EAAT2 at approximately 62KD. The expected band size for EAAT2 is at 62KD.
SLC1A2 / EAAT2 / GLT-1 Antibody - EAAT2 antibody Western blot. All lanes: Anti EAAT2 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: U87 Whole Cell Lysate at 40 ug. Lane 4: SMMC Whole Cell Lysate at 40 ug. Lane 5: PANC Whole Cell Lysate at 40 ug. Lane 6: A549 Whole Cell Lysate at 40 ug. Predicted band size: 62 kD. Observed band size: 62 kD.
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Polyclonal Rabbit anti‑Human SLC1A2 / EAAT2 / GLT‑1 Antibody (aa461‑574, WB) LS‑C357612

Polyclonal Rabbit anti‑Human SLC1A2 / EAAT2 / GLT‑1 Antibody (aa461‑574, WB) LS‑C357612

Note: This antibody replaces LS-C388383
Antibody:
SLC1A2 / EAAT2 / GLT-1 Rabbit anti-Human Polyclonal (aa461-574) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357612-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SLC1A2 / EAAT2 / GLT-1 Rabbit anti-Human Polyclonal (aa461-574) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GLT-1 antibody LS-C357612 is an unconjugated rabbit polyclonal antibody to GLT-1 (SLC1A2 / EAAT2) (aa461-574) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human SLC1A2 / EAAT2 / GLT-1
Synonyms
SLC1A2 | EAAT-2 | GLT-1 | Glutamate transporter 1 | EAAT2 | GLT1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human EAAT2 recombinant protein (Position: T461-K574). Human EAAT2 shares 96% amino acid (aa) sequence identity with both mouse and rat EAAT2.
Epitope
aa461-574
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC1A2 / EAAT2 / GLT-1
P43004 NM_004171 NP_004162.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/20/2024