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Catalog Number Size Price
LS-C776957-100 100 µg (1 mg/ml) $478 
SHANK3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK1/SHANK3 Antibody. (D) Composite.
SHANK3 Antibody - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.
SHANK3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK1/SHANK3 Antibody. (D) Composite.
SHANK3 Antibody - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.
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Monoclonal Mouse anti‑Human SHANK3 Antibody (clone S367‑51, APC, IHC, IF, WB) LS‑C776957

Monoclonal Mouse anti‑Human SHANK3 Antibody (clone S367‑51, APC, IHC, IF, WB) LS‑C776957

Antibody:
SHANK3 Mouse anti-Human Monoclonal (APC) (S367-51) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$478
LS-C776957-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SHANK3 Mouse anti-Human Monoclonal (APC) (S367-51) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
SHANK3 antibody LS-C776957 is an APC-conjugated mouse monoclonal antibody to SHANK3 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human SHANK3
Synonyms
SHANK3 | DEL22q13.3 | KIAA1650 | SPANK-2 | PROSAP2 | PSAP2 | SCZD15
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
S367-51
Conjugations
Allophycocyanin. Also available conjugated with AP, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
Specificity
Detects ~190 kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
Applications
  • IHC
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SHANK3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/24/2024