Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
RAPGEF5, GFR, KIAA0277, M-Ras-regulated Rap GEF, M-Ras-regulated GEF, MRGEF, Related to Epac, REPAC, MR-GEF
(tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Western blot (1:500 - 1:3000)
RAPGEF5 / GFR antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 179 of Human RAPGEF5. Percent identity by BLAST analysis: Human (100%); Monkey (98%).
Human RAPGEF5 / GFR
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).