Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > RAF1 / RAF > LS-C661975
 
Print Page  
Catalog Number Size Price
LS-C661975-10 10 µg $318 
LS-C661975-100 100 µg $470 
RAF1 / RAF Antibody - Western blot analysis of Raf1 using anti-Raf1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,Lane 2: mouse kidney tissue lysate,Lane 3: human MCF-7 whole cell lysate,Lane 4: human 22RV1 whole cell lysate,Lane 5: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raf1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Raf1 at approximately 73KD. The expected band size for Raf1 is at 73KD.
RAF1 / RAF Antibody - Flow Cytometry analysis of HeLa cells using anti-Raf1 antibody. Overlay histogram showing HeLa cells stained with PB10089 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Raf1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
RAF1 / RAF Antibody - Western blot analysis of Raf1 using anti-Raf1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,Lane 2: mouse kidney tissue lysate,Lane 3: human MCF-7 whole cell lysate,Lane 4: human 22RV1 whole cell lysate,Lane 5: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raf1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Raf1 at approximately 73KD. The expected band size for Raf1 is at 73KD.
RAF1 / RAF Antibody - Flow Cytometry analysis of HeLa cells using anti-Raf1 antibody. Overlay histogram showing HeLa cells stained with PB10089 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Raf1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (WB) LS‑C661975

Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (WB) LS‑C661975

Submit Review LSBio Guarantee
Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661975-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RAF antibody LS-C661975 is an unconjugated rabbit polyclonal antibody to human RAF (RAF1). Validated for WB.
Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human Raf1 recombinant protein (Position: Y364-Q451). Human Raf1 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Raf1.
Specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

Publications (0)

Submit a Publication for LS-C661975

Customer Reviews (0)

Submit a review for LS-C661975

Featured Products

RAF1 / RAF Antibody - Anti-RAF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ RAF1 / RAF Antibody (N‑Terminus) LS‑B6239
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
RAF1 / RAF Antibody - IHC of paraffin-embedded liver cancer tissues using RAF1 mouse monoclonal antibody with DAB staining.
RAF1 / RAF Antibody (clone 4G4) LS‑C169472
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
RAF1 / RAF Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
RAF1 / RAF Antibody (aa1‑30) LS‑C97203
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
Product
RAF1 / RAF Antibody (aa256‑286, Biotin) LS‑C281924
Species: Human
Applications: IHC, Western blot, ELISA
RAF1 / RAF Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ RAF1 / RAF Antibody (C‑Terminus) LS‑B14835
Species: Human, Mouse, Rat, Bovine, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
RAF1 / RAF Antibody (Pro302) LS‑C9879
Species: Human, Monkey
Applications: Western blot, Immunoprecipitation
See all RAF1 / RAF Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com