Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C370062-50 50 µg (2.815 mg/ml) $294 
LS-C370062-100 100 µg $360 
NPHS1 / Nephrin Antibody - Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NPHS1 / Nephrin Antibody - Immunohistochemistry of paraffin-embedded human kidney tissue using NPHS1 Antibody at dilution of 1:100
NPHS1 / Nephrin Antibody - Immunofluorescence staining of HepG2 cells with NPHS1 Antibody at 1:125, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
NPHS1 / Nephrin Antibody - Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NPHS1 antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 135, 131 kDa Observed band size: 135 kDa
NPHS1 / Nephrin Antibody - Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NPHS1 / Nephrin Antibody - Immunohistochemistry of paraffin-embedded human kidney tissue using NPHS1 Antibody at dilution of 1:100
NPHS1 / Nephrin Antibody - Immunofluorescence staining of HepG2 cells with NPHS1 Antibody at 1:125, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
NPHS1 / Nephrin Antibody - Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: NPHS1 antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 135, 131 kDa Observed band size: 135 kDa
1 of 4
2 of 4
3 of 4
4 of 4

Polyclonal Rabbit anti‑Human NPHS1 / Nephrin Antibody (aa23‑257, IHC) LS‑C370062

Polyclonal Rabbit anti‑Human NPHS1 / Nephrin Antibody (aa23‑257, IHC) LS‑C370062

Antibody:
NPHS1 / Nephrin Rabbit anti-Human Polyclonal (aa23-257) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C370062-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NPHS1 / Nephrin Rabbit anti-Human Polyclonal (aa23-257) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Nephrin antibody LS-C370062 is an unconjugated rabbit polyclonal antibody to human Nephrin (NPHS1) (aa23-257). Validated for ELISA and IHC.
Target
Human NPHS1 / Nephrin
Synonyms
NPHS1 | Nephrin | NPHN | CNF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human Nephrin protein (aa23-257).
Epitope
aa23-257
Specificity
Human NPHS1 / Nephrin
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NPHS1 / Nephrin
O60500 NM_004646 NP_004637.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/30/2024