Human NOS3 / eNOS
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
KLH-conjugated, synthetic peptide (C-RIRTQ[ pS]FSLQER) corresponding to aa1172-1183 of human eNOS (endothelial nitric oxide synthase) with an N-Terminus cysteine added.
Recognizes phosphorylated eNOS, Mr 132kD. Additional unknown bands at 50 and 64kD Species cross-reactivity: Human; other species not tested. Species sequence homology: rat, mouse, bovine, porcine and canine.
Suitable for use in Western Blot. Western Blot: 1:1000 dilution detects recombinant eNOS phosphorylated by Akt. Recombinant eNOS and phosphorylated recombinant eNOS were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-eNOS (Ser1177), 1:1000 dilution. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.05% Sodium Azide, 30% Glycerol
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.