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Catalog Number Size Price
LS-C23072-100 100 µl $734 
Antibody

Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (phospho‑Ser1177, WB) LS‑C23072

Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (phospho‑Ser1177, WB) LS‑C23072

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$734
LS-C23072-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
ENOS antibody LS-C23072 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (pSer1177) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide (C-RIRTQ[ pS]FSLQER) corresponding to aa1172-1183 of human eNOS (endothelial nitric oxide synthase) with an N-Terminus cysteine added.
Epitope
pSer1177
Specificity
Recognizes phosphorylated eNOS, Mr 132kD. Additional unknown bands at 50 and 64kD Species cross-reactivity: Human; other species not tested. Species sequence homology: rat, mouse, bovine, porcine and canine.
Applications
  • Western blot (1:1000)
Usage
Suitable for use in Western Blot. Western Blot: 1:1000 dilution detects recombinant eNOS phosphorylated by Akt. Recombinant eNOS and phosphorylated recombinant eNOS were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-eNOS (Ser1177), 1:1000 dilution. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022