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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C744636-25 25 µl (80 mg/ml) $235 
RELA / NFKB p65 Antibody - Immunohistochemistry of NFKB p65 (Rel A) antibody. Tissue: lymphocytes and germinal center cells of the tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: NFKB p65 (Rel A) antibody at 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Moderate positive nuclear or cytoplasmic staining was observed in lymphocytes and germinal center cells of the tonsil.
RELA / NFKB p65 Antibody - Western blot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
RELA / NFKB p65 Antibody - Western Blot of rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell extract. Lane 2: HeLa cell extract. Load: 35 µg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None.
RELA / NFKB p65 Antibody - Immunohistochemistry of NFKB p65 (Rel A) antibody. Tissue: lymphocytes and germinal center cells of the tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: NFKB p65 (Rel A) antibody at 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Moderate positive nuclear or cytoplasmic staining was observed in lymphocytes and germinal center cells of the tonsil.
RELA / NFKB p65 Antibody - Western blot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
RELA / NFKB p65 Antibody - Western Blot of rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell extract. Lane 2: HeLa cell extract. Load: 35 µg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None.
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Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (C‑Terminus, IHC, WB) LS‑C744636

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (C‑Terminus, IHC, WB) LS‑C744636

Antibody:
Rabbit Polyclonal to Human RELA / NFKB p65
Application:
IHC, WB, ELISA, GS
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C744636-25
25 µl
80 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human RELA / NFKB p65
Application:
IHC, WB, ELISA, GS
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-C744636 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, GS, IHC and WB.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC (1:400)
  • Western blot (1:2000 - 1:5000)
  • ELISA (1:5000)
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
C-Terminus
Usage
Applications should be user optimized.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

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Requested From: United States
Date Requested: 8/6/2020