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Antibodies > NALP3 / NLRP3 > LS-C312821
 
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Catalog Number Size Price
LS-C312821-10 10 µg $318 
LS-C312821-100 100 µg (0.5 mg/ml) $470 
NALP3 / NLRP3 Antibody - IHC analysis of NLRP3 using anti-NLRP3 antibody. NLRP3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NLRP3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody. IHC(P): Human Tonsil Tissue.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat colon tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in immunocytochemical section of THP-1 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in immunocytochemical section of THP-1 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat colon tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - WB of NALP3 / NLRP3 antibody. All lanes: Anti-NLRP3 at 0.5ug/ml. Lane 1: HEP-2 Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: U87 Whole Cell Lysate at 40ug. Lane 4: CEM Whole Cell Lysate at 40ug. Predicted bind size: 118KD. Observed bind size: 118KD.
NALP3 / NLRP3 Antibody - Flow Cytometry analysis of U937 cells using anti-CIAS1/NALP3 antibody. Overlay histogram showing U937 cells stained with anti-CIAS1/NALP3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIAS1/NALP3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
NALP3 / NLRP3 Antibody - Flow Cytometry analysis of THP-1 cells using anti-CIAS1/NALP3 antibody. Overlay histogram showing THP-1 cells stained with anti-CIAS1/NALP3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIAS1/NALP3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
NALP3 / NLRP3 Antibody - IHC analysis of NLRP3 using anti-NLRP3 antibody. NLRP3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NLRP3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody. IHC(P): Human Tonsil Tissue.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat colon tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in immunocytochemical section of THP-1 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in immunocytochemical section of THP-1 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat colon tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - IF analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody CIAS1/NALP3 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CIAS1/NALP3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NALP3 / NLRP3 Antibody - WB of NALP3 / NLRP3 antibody. All lanes: Anti-NLRP3 at 0.5ug/ml. Lane 1: HEP-2 Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: U87 Whole Cell Lysate at 40ug. Lane 4: CEM Whole Cell Lysate at 40ug. Predicted bind size: 118KD. Observed bind size: 118KD.
NALP3 / NLRP3 Antibody - Flow Cytometry analysis of U937 cells using anti-CIAS1/NALP3 antibody. Overlay histogram showing U937 cells stained with anti-CIAS1/NALP3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIAS1/NALP3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
NALP3 / NLRP3 Antibody - Flow Cytometry analysis of THP-1 cells using anti-CIAS1/NALP3 antibody. Overlay histogram showing THP-1 cells stained with anti-CIAS1/NALP3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIAS1/NALP3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human NALP3 / NLRP3 Antibody (aa12‑31, IHC, WB) LS‑C312821

Polyclonal Rabbit anti‑Human NALP3 / NLRP3 Antibody (aa12‑31, IHC, WB) LS‑C312821

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Note: This antibody replaces LS-C388950
Antibody:
NALP3 / NLRP3 Rabbit anti-Human Polyclonal (aa12-31) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312821-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NALP3 / NLRP3 Rabbit anti-Human Polyclonal (aa12-31) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
NLRP3 antibody LS-C312821 is an unconjugated rabbit polyclonal antibody to NLRP3 (NALP3) (aa12-31) from human. It is reactive with human and monkey. Validated for IHC and WB.
Target
Human NALP3 / NLRP3
Synonyms
NLRP3 | AGTAVPRL | AII | C1orf7 | Caterpiller protein 1.1 | Cryopyrin | FCAS | FCU | PYPAF1 | NALP3 | CIAS1 | CLR1.1 | MWS
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human CIAS1(12-31 aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.
Epitope
aa12-31
Specificity
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NALP3 / NLRP3
Q96P20 NM_004895 NP_004886.3

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Requested From: United States
Date Requested: 4/26/2024
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