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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154000-100 100 µg (1 mg/ml) $325 
DYKDDDDK Tag Antibody - Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Monoclonal Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot.
DYKDDDDK Tag Antibody - Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 ul. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
DYKDDDDK Tag Antibody - Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Monoclonal Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot.
DYKDDDDK Tag Antibody - Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 ul. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
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Monoclonal Mouse DYKDDDDK Tag Antibody (clone 29E4.G7, IHC, WB) LS‑C154000

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 29E4.G7, IHC, WB) LS‑C154000

Antibody:
Mouse Monoclonal to DYKDDDDK Tag
Application:
IHC, WB, Flo, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C154000-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to DYKDDDDK Tag
Application:
IHC, WB, Flo, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C154000 is an unconjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ELISA, Flow, IHC and WB.
Host
Mouse
Clonality
IgG2a,k Monoclonal
Clone
29E4.G7
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:2000 - 1:20000)
  • Flow Cytometry
  • ELISA (1:150000 - 1:250000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Specificity
This monoclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Usage
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 6/2/2020