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Antibodies > DYKDDDDK Tag > LS-C413444
 
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Catalog Number Size Price
LS-C413444-100 100 µl (1 mg/ml) $267 
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody - Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate.
DYKDDDDK Tag Antibody - Immunoprecipitation of FLAG-tagged protein from HEK293T cells transfected with vector overexpressing Flag tag; using Anti-FLAG-tag Antibody.
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody - Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate.
DYKDDDDK Tag Antibody - Immunoprecipitation of FLAG-tagged protein from HEK293T cells transfected with vector overexpressing Flag tag; using Anti-FLAG-tag Antibody.
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Monoclonal Mouse DYKDDDDK Tag Antibody (IF, WB) LS‑C413444

Monoclonal Mouse DYKDDDDK Tag Antibody (IF, WB) LS‑C413444

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Antibody:
DYKDDDDK Tag Mouse Monoclonal Antibody
Application:
ICC, IF, WB, IP
Format:
Unconjugated, Unmodified
Price
Catalog Number
$267
LS-C413444-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Mouse Monoclonal Antibody
Application:
ICC, IF, WB, IP
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C413444 is an unconjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ICC, IF, IP and WB.
Target
DYKDDDDK Tag
Host
Mouse
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag.
Specificity
Recognizes C-terminal, internal, and N-terminal FLAG-tag fusion proteins.
Applications
  • ICC (1:200 - 1:500)
  • Immunofluorescence (1:200 - 1:500)
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation (1:100 - 1:200)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
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DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
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Dermatopontin / DPT Antibody - DPT / Dermatopontin antibody Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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