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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DYKDDDDK Tag

Enterokinase (ECS) Cleavage Site / DYKDDDDK Tag


DYKDDDDK Tag Target Details

Target Name: Enterokinase (ECS) Cleavage Site / DYKDDDDK Tag

Publications (7)

1
Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. Journal of immunological methods. 2008 331:27-38. [PubMed:18054954] [PMC:PMC2864634]
2
Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo. Idoyaga J, Cheong C, Suda K, Suda N, Kim JY, Lee H, Park CG, Steinman RM. Journal of immunology (Baltimore, Md. : 1950). 2008 180:3647-50. [PubMed:18322168]
3
MCM proteins are negative regulators of hypoxia-inducible factor 1. Hubbi ME, Luo W, Baek JH, Semenza GL. Molecular cell. 2011 42:700-12. (WB, IP; Human) [PubMed:21658608] [PMC:PMC3131976]
4
Regulation of the surface expression of 42 GABAA receptors by high efficacy states. Kuver A, Shen H, Smith SS. Brain research. 2012 1463:20-Jan. (WB; Human) [PubMed:22609410] [PMC:PMC3371167]
5
Differentiated embryo chondrocyte 1 (DEC1) represses PPAR2 gene through interacting with CCAAT/enhancer binding protein (C/EBP). Park YK, Park H. Molecules and cells. 2012 33:575-81. (WB; Mouse) [PubMed:22610404]
6
ASXL1 mutations promote myeloid transformation through loss of PRC2-mediated gene repression. Abdel-Wahab O, Adli M, LaFave LM, Gao J, Hricik T, Shih AH, Pandey S, Patel JP, Chung YR, Koche R, Perna F, Zhao X, Taylor JE, Park CY, Carroll M, Melnick A, Nimer SD, Jaffe JD, Aifantis I, Bernstein BE, Levine RL. Cancer cell. 2012 22:180-93. (IP; Human) [PubMed:22897849] [PMC:PMC3422511]
7
Embryonic Stem Cell-Based Modeling of Tau Pathology in Human Neurons. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. The American journal of pathology. 2013 [PubMed:23499461] Related Antibodies: LS-C157769.
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Filtered by: None
Target
DYKDDDDK Tag (105)
Reactivity
Human (1)
Mouse (5)
All species (18)
Synthetic (6)
Application
IHC (14)
IHC-P (2)
WB (93)
Flo (7)
DB (3)
ELISA (54)
ICC (12)
IF (17)
IP (24)
Micro (1)
Peptide-ELISA (1)
Host
rabbit (50)
mouse (36)
goat (5)
chicken (5)
rat (9)
Isotype
IgG (37)
IgG1 (7)
IgG1,k (3)
IgG2a (5)
IgG2b (6)
IgG2b,k (7)
IgY (3)
Clonality
monoclonal mc (48)
polyclonal pc (57)
Clone
114F12C8 (3)
1557CT661.18.1 (1)
1A8 (1)
29E4.G7 (5)
29E4.G7.H11 (1)
2F11G1 (1)
5A8E5 (4)
5E3C8 (1)
6F7 (4)
FG4R (2)
GST.B6 (1)
IE6 (1)
L5 (5)
M2 (4)
NYRFLAG (1)
Format
Agarose Conjugated (1)
AP Conjugated (4)
APC Conjugated (1)
Atto 390 Conjugated (1)
Atto 488 Conjugated (1)
Atto 565 Conjugated (1)
Atto 594 Conjugated (2)
Atto 633 Conjugated (1)
Atto 655 Conjugated (1)
Atto 680 Conjugated (1)
Atto 700 Conjugated (1)
Azide-free (1)
Biotin Conjugated (8)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
Europium Conjugated (1)
FITC Conjugated (6)
HiLyte Fluor 488 Conjugated (1)
HiLyte Fluor 555 Conjugated (1)
HiLyte Fluor 647 Conjugated (1)
HRP Conjugated (13)
PE (1)
PE Conjugated (1)
PerCP Conjugated (1)
RPE Conjugated (3)
SA Conjugated (1)
Unconjugated (50)
Epitope
C-Terminus (11)
N-Terminus (5)
Publications
No (104)
Yes (1)
Showing 1-25 of 105 results

DYKDDDDK Tag Antibody (N-Terminus, clone L5) LS-C142551
Rat Monoclonal [clone L5] (IgG2a)
ICC, IF, IP, WB
Unconjugated
500µl  $335.00
DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DYKDDDDK Tag Antibody (N-Terminus, clone L5, Biotin) LS-C143064
Rat Monoclonal [clone L5] (IgG2a)
ICC, IF, IP, WB
Biotin Conjugated
50µl 1.18mg/ml  $365.00
DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

DYKDDDDK Tag Antibody (clone 5E3C8) LS-C51639
Mouse Monoclonal [clone 5E3C8] (IgG1)
ELISA, WB
Unconjugated
40µg 0.5mg/ml  $245.00
Western blot of Enterokinase protein using Enterokinase Antibody, mAb, Mouse (Enterokinase Antibody, mAb, Mouse, 1 ug/ml) The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit Predicted Size: 38 kD Observed Size: 38 kD

DYKDDDDK Tag Antibody LS-C129100
Chicken Polyclonal (IgY)
ELISA, WB
Unconjugated
100µg 1mg/ml  $455.00

DYKDDDDK Tag Antibody LS-C413444
Mouse Monoclonal
ICC, IF, IP, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate.

DYKDDDDK Tag Antibody (clone 5A8E5, FITC) LS-C203622
Mouse Monoclonal [clone 5A8E5] (IgG2b,k)
Flo
FITC Conjugated
100µg 0.5mg/ml  $285.00
Flow cytometric analysis of transfected HEK-293 cell expressing DYKDDDDK-tagged protein using THE TM Anti-DYKDDDDK [FITC] Monoclonal Antibody or with a negative control antibody (open histogram).

DYKDDDDK Tag Antibody (clone 6F7) LS-C188601
Rat Monoclonal [clone 6F7] (IgG1) to Synthetic DYKDDDDK Tag
Synthetic
ELISA, Flo, IF, IHC-P, IP, WB
Unconjugated
25µg  $195.00; 100µg 1mg/ml  $285.00; 0.2mg  $355.00

DYKDDDDK Tag Antibody LS-C157769
Publications (1)
Publications (1)
Rabbit Polyclonal
WB
Unconjugated
200µl  $325.00
Western blot of lysate from 12tag protein, using FLAG tag antibody (DYKDDDDK). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from 12tag recombinant protein, using FLAG tag antibody (DYKDDDDK). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

DYKDDDDK Tag Antibody (C-Terminus) LS-C154223
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
Unconjugated
250µg 1mg/ml  $295.00
Antibody for the detection of FLAG conjugated proteins - Western Blot. antibody to detect FLAG conjugated proteins is shown to detect as little as 3 ng of amino-terminal FLAG tagged recombinant protein by western blot. This antibody was used at a 1:1000 dilution to detect 3-fold serial dilutions of amino-terminal FLAG-Bacterial Alkaline Phosphatase (BAP) fusion protein (Sigma P-7582) starting at 1.0 ug of protein as shown in lanes 1-6 respectively. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

DYKDDDDK Tag Antibody (Biotin) LS-C65175
Rabbit Polyclonal
ELISA, IHC, WB
Biotin Conjugated
100µg  $740.00

DYKDDDDK Tag Antibody (clone 1557CT661.18.1) LS-C392574
Mouse Monoclonal [clone 1557CT661.18.1] (IgG1,k)
Peptide-ELISA, WB
Unconjugated
200µl  $325.00
Anti-FLAG Tag Antibody at 1:2000 dilution + 12tag protein lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

DYKDDDDK Tag Antibody (C-Terminus, clone GST.B6) LS-C47237
Mouse Monoclonal [clone GST.B6] (IgG2b)
IP, WB
Unconjugated
100µg 1mg/ml  $275.00
Western blot Image

DYKDDDDK Tag Antibody LS-C83260
Goat Polyclonal
ELISA, ICC, IP, WB
Unconjugated
100µg 1mg/ml  $355.00; 1mg  $1,140.00
Detection of ECS-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein

DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS-C154016
Mouse Monoclonal [clone 29E4.G7] (IgG)
ELISA, IHC, WB
HRP Conjugated
100µg 1mg/ml  $365.00

DYKDDDDK Tag Antibody (clone 114F12C8) LS-C742067
Rabbit Monoclonal [clone 114F12C8] (IgG)
ELISA, IP, WB
Unconjugated
40µg  $325.00
Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit. Loading: Lane 1: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 2: Rabbit IgG (4 µg)++ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input) Lane 4: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 5: Rabbit IgG (4 µg)++ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211)
Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit to DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Lane 5: C-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 6: C-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 7: C-terminal DYKDDDDK-tagged fusion protein Lane 8: C-terminal DYKDDDDK-tagged fusion protein (1 ng) Lane 9: Internal positions DYKDDDDK-tagged fusion protein (50 ng) (25 ng) Lane 11: Internal positions DYKDDDDK-tagged fusion protein (10 ng) Lane 12: Internal positions DYKDDDDK-tagged fusion protein (5 ng) Lane 13: Internal positions DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211) (Licor,926-32211)

DYKDDDDK Tag Antibody (clone 6F7, HRP) LS-C188602
Rat Monoclonal [clone 6F7] (IgG1) to Synthetic DYKDDDDK Tag
Synthetic
ELISA, IHC-P, WB
HRP Conjugated
0.1mg  $425.00
DYKDDDDK tagged protein probed with Rat anti-DYKDDDDK tag:HRP This image was taken for the unconjugated form of this product. Other forms have not been tested.

DYKDDDDK Tag Antibody (clone 114F12C8, HRP) LS-C742068
Rabbit Monoclonal [clone 114F12C8] (IgG)
ELISA, WB
HRP Conjugated
40µg  $325.00
Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody [HRP], mAb, Rabbit to N-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody [HRP], mAb, Rabbit 1 µg/ml
Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody [HRP], mAb, Rabbit to C-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: C-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: C-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: C-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: C-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody [HRP], mAb, Rabbit 1 µg/ml

DYKDDDDK Tag Antibody (clone 114F12C8, Biotin) LS-C742069
Rabbit Monoclonal [clone 114F12C8] (IgG)
ELISA, WB
Biotin Conjugated
40µg  $325.00
Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit to N-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit 1 µg/ml Secondary Antibody: Streptavidin-HRP(M00091,15L000972) 0.2 µg/ml
Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit to C-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: C-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: C-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: C-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: C-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit 1 µg/ml Secondary Antibody: Streptavidin-HRP(M00091,15L000972) 0.2 µg/ml

DYKDDDDK Tag Antibody (clone M2) LS-C758166
Mouse Monoclonal [clone M2] (IgG) to Mouse DYKDDDDK Tag
Mouse
ELISA, Flo, IF, Micro
Unconjugated
100µg  $415.00

DYKDDDDK Tag Antibody (clone 5A8E5) LS-C203597
Mouse Monoclonal [clone 5A8E5] (IgG2b,k)
ELISA, IF, IP, WB
Unconjugated
100µg  $275.00; 200µg  $375.00
293 cells transfected with N-terminal DYKDDDDK tag protein. Primary antibody: 1 ug/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ug/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).
293 cells transfected with C-terminal DYKDDDDK tag protein. Primary antibody: 1 ?g/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ?g/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).

DYKDDDDK Tag Antibody LS-C40549
Rabbit Polyclonal (IgG)
WB
Unconjugated
1ea  $345.00

DYKDDDDK Tag Antibody (HRP) LS-C129103
Chicken Polyclonal (IgY)
ELISA, WB
HRP Conjugated
100µg  $465.00

DYKDDDDK Tag Antibody LS-C129107
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
Unconjugated
50µl  $385.00

DYKDDDDK Tag Antibody LS-C195187
Rabbit Polyclonal (IgG)
Human
DB, ELISA, IHC, WB
Unconjugated
500µl  $315.00; 1ml  $425.00

DYKDDDDK Tag Antibody LS-C51791
Rabbit Polyclonal
ELISA, IHC, IP, WB
Unconjugated
200µg 1000µl 0.2mg/ml  $295.00
Showing 1-25 of 105 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).