Products
Services
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

DYKDDDDK Tag

Enterokinase (ECS) Cleavage Site / DYKDDDDK Tag


DYKDDDDK Tag Target Details

Target Name: Enterokinase (ECS) Cleavage Site / DYKDDDDK Tag

Publications (7)

1
Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. Journal of immunological methods. 2008 331:27-38. [PubMed:18054954] [PMC:PMC2864634]
2
Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo. Idoyaga J, Cheong C, Suda K, Suda N, Kim JY, Lee H, Park CG, Steinman RM. Journal of immunology (Baltimore, Md. : 1950). 2008 180:3647-50. [PubMed:18322168]
3
MCM proteins are negative regulators of hypoxia-inducible factor 1. Hubbi ME, Luo W, Baek JH, Semenza GL. Molecular cell. 2011 42:700-12. (WB, IP; Human) [PubMed:21658608] [PMC:PMC3131976]
4
Regulation of the surface expression of 42 GABAA receptors by high efficacy states. Kuver A, Shen H, Smith SS. Brain research. 2012 1463:20-Jan. (WB; Human) [PubMed:22609410] [PMC:PMC3371167]
5
Differentiated embryo chondrocyte 1 (DEC1) represses PPAR2 gene through interacting with CCAAT/enhancer binding protein (C/EBP). Park YK, Park H. Molecules and cells. 2012 33:575-81. (WB; Mouse) [PubMed:22610404]
6
ASXL1 mutations promote myeloid transformation through loss of PRC2-mediated gene repression. Abdel-Wahab O, Adli M, LaFave LM, Gao J, Hricik T, Shih AH, Pandey S, Patel JP, Chung YR, Koche R, Perna F, Zhao X, Taylor JE, Park CY, Carroll M, Melnick A, Nimer SD, Jaffe JD, Aifantis I, Bernstein BE, Levine RL. Cancer cell. 2012 22:180-93. (IP; Human) [PubMed:22897849] [PMC:PMC3422511]
7
Embryonic Stem Cell-Based Modeling of Tau Pathology in Human Neurons. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. The American journal of pathology. 2013 [PubMed:23499461] Related Antibodies: LS-C157769.
more


DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rat Monoclonal [clone L5] (IgG2a)
ICC, IF, IP, WB
Biotin Conjugated
50µl   1.18mg/ml   $365.00
DYKDDDDK Tag Antibody - Western blot of Enterokinase protein using Enterokinase Antibody, mAb, Mouse (Enterokinase Antibody, mAb, Mouse, 1 ug/ml) The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit Predicted Size: 38 kD Observed Size: 38 kD
Select
Mouse Monoclonal [clone 5E3C8] (IgG1)
ELISA, WB
Unconjugated
40µg   0.5mg/ml   $245.00
DYKDDDDK Tag Antibody - Flow cytometric analysis of transfected HEK-293 cell expressing DYKDDDDK-tagged protein using THE TM Anti-DYKDDDDK [FITC] Monoclonal Antibody or with a negative control antibody (open histogram).
Select
Mouse Monoclonal [clone 5A8E5] (IgG2b,k)
Flo
FITC Conjugated
100µg   $285.00
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Select
Mouse Monoclonal
ICC, IF, IP, WB
Unconjugated
100µl   1mg/ml   $275.00
DYKDDDDK Tag Antibody - Anti-FLAG Tag Antibody at 1:2000 dilution + 12tag protein lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Select
Mouse Monoclonal [clone 1557CT661.18.1] (IgG1,k)
Peptide-ELISA, WB
Unconjugated
200µl   $325.00
Select
Chicken Polyclonal (IgY)
ELISA, WB
Unconjugated
100µg   1mg/ml   $455.00
Select
Rat Monoclonal [clone 6F7] (IgG1) to Synthetic DYKDDDDK Tag
Synthetic
ELISA, Flo, IF, IHC-P, IP, WB
Unconjugated
25µg   $205.00; 0.1mg   1mg/ml   $295.00; 0.2mg   $365.00
DYKDDDDK Tag Antibody - Detection of ECS-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein
Select
Goat Polyclonal
ELISA, ICC, IP, WB
Unconjugated
100µg   1mg/ml   $355.00; 1mg   $1,170.00
DYKDDDDK Tag Antibody - Western blot of lysate from 12tag protein, using FLAG tag antibody (DYKDDDDK). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Select
Rabbit Polyclonal
WB
Unconjugated
200µl   $325.00
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. antibody to detect FLAG conjugated proteins is shown to detect as little as 3 ng of amino-terminal FLAG tagged recombinant protein by western blot. This antibody was used at a 1:1000 dilution to detect 3-fold serial dilutions of amino-terminal FLAG-Bacterial Alkaline Phosphatase (BAP) fusion protein (Sigma P-7582) starting at 1.0 ug of protein as shown in lanes 1-6 respectively. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Select
Rabbit Polyclonal (IgG)
ELISA, IHC, WB
Unconjugated
250µg   1mg/ml   $295.00
Select
Rabbit Polyclonal
ELISA, IHC, WB
Biotin Conjugated
100µg   $740.00
DYKDDDDK Tag Antibody - Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit. Loading: Lane 1: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 2: Rabbit IgG (4 µg)++ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input) Lane 4: MonoRabTM DYKDDDDK antibody (4 µg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 5: Rabbit IgG (4 µg)++ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody, mAb, Rabbit 0.2 µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211)
Select
Rabbit Monoclonal [clone 114F12C8] (IgG)
ELISA, IP, WB
Unconjugated
40µg   $325.00
DYKDDDDK Tag Antibody
Select
Mouse Monoclonal [clone GST.B6] (IgG2b)
IP, WB
Unconjugated
100µg   1mg/ml   $275.00
DYKDDDDK Tag Antibody - 293 cells transfected with N-terminal DYKDDDDK tag protein. Primary antibody: 1 ug/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ug/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).
Select
Mouse Monoclonal [clone 5A8E5] (IgG2b,k)
ELISA, IF, IP, WB
Unconjugated
100µg   $275.00; 200µg   $375.00
DYKDDDDK Tag Antibody - Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 ul. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
Select
Mouse Monoclonal [clone 29E4.G7] (IgG2a,k)
ELISA, Flo, IHC, WB
Unconjugated
100µg   1mg/ml   $325.00
Select
Mouse Monoclonal (IgG)
ELISA, IHC, WB
HRP Conjugated, Azide-free
0.1mg   $415.00
DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
Select
Mouse Monoclonal [clone 29E4.G7] (IgG2a,k)
ELISA, IHC, WB
HRP Conjugated
100µg   1mg/ml   $365.00
DYKDDDDK Tag Antibody - DYKDDDDK tagged protein probed with Rat anti-DYKDDDDK tag:HRP This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rat Monoclonal [clone 6F7] (IgG1) to Synthetic DYKDDDDK Tag
Synthetic
ELISA, IHC-P, WB
HRP Conjugated
0.1mg   $425.00
DYKDDDDK Tag Antibody - Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody [HRP], mAb, Rabbit to N-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody [HRP], mAb, Rabbit 1 µg/ml
Select
Rabbit Monoclonal [clone 114F12C8] (IgG)
ELISA, WB
HRP Conjugated
40µg   $325.00
DYKDDDDK Tag Antibody - Sensitivity and specificity of MonoRab?? DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit to N-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4: N-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab?? DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit 1 µg/ml Secondary Antibody: Streptavidin-HRP(M00091,15L000972) 0.2 µg/ml
Select
Rabbit Monoclonal [clone 114F12C8] (IgG)
ELISA, WB
Biotin Conjugated
40µg   $325.00
Select
Mouse Monoclonal [clone M2] (IgG) to Mouse DYKDDDDK Tag
Mouse
ELISA, Flo, IF, Micro
Unconjugated
100µg   $425.00
Select
Rabbit Polyclonal (IgG)
WB
Unconjugated
1ea   $345.00
DYKDDDDK Tag Antibody - Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 µL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG™ antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
Select
Mouse Monoclonal [clone 29E4.G7] (IgG2a,k)
ELISA, Flo, IHC, WB
Unconjugated
500µg   1mg/ml   $485.00
Select
Mouse Monoclonal [clone 29E4.G7] (IgG2a,k)
ELISA, Flo, IHC, WB
Biotin Conjugated
100µg   1mg/ml   $425.00
Select
Mouse Monoclonal (IgG1)
ELISA, IF, IP, WB
Unconjugated
50µl   $385.00
Viewing 1-25 of 103 products



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).