Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Anti-DYKDDDDK Affinity Gel binds the FLAG® epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide is recommended for competitive elution to recover fusion protein (see protocol).
This agarose-conjugated antibody is optimally suited for immunoprecipitation and purification of DYKDDDDK tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. DYKDDDDK tag can be cleaved by the enterokinase because it contains the Enterokinase Cleavage Site (ECS). DO NOT USE A MAGNETIC STIR BAR WITH THIS PRODUCT. Do not use chaotropic reagents such as guanidine HCl; however urea may be used at less than 1 M concentration. Do not use any reagent that contains reducing agents such as DTT, DTE, or 2-mercaptoethanol with this product. Do not exceed 5% concentration of TWEEN 20 or TRITON X-100 with this product. Do not exceed 0.1% IGEPAL CA-630 or CHAPS. Do not exceed 0.2% digitonin. Do not exceed 1.0 M sodium chloride. DO NOT USE deoxycholate with this product. DO NOT USE SDS. Keep exposure to glycine HCl to less than 20 minutes.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
For research use only. Intended for use by laboratory professionals.
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