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Mouse Anti‑DYKDDDDK Tag Secondary Antibody (clone 29E4.G7) LS‑C154012

Catalog Number Size Price
LS-C154012-500 500 µg (1 mg/ml) $485 
Antibody

Mouse Anti‑DYKDDDDK Tag Secondary Antibody (clone 29E4.G7) LS‑C154012

Antibody:
Mouse Monoclonal to DYKDDDDK Tag
Application:
IHC, WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-C154012-500
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to DYKDDDDK Tag
Application:
IHC, WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C154012 is an unconjugated mouse monoclonal antibody. Validated for ELISA, IHC and WB.
Host
Mouse
Clonality
IgG Monoclonal
Clone
29E4.G7
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:2000 - 1:20000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Specificity
The purified antibody is directed against DYKDDDDK and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal antibody detects over-expressed proteins containing tDYKDDDDK. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Usage
This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product
Species:
Applications: IHC, Western blot, ELISA
Western blot Image
Species:
Applications: Western blot, Immunoprecipitation
Product
Species:
Applications: Western blot, ELISA

Requested From: United States
Date Requested: 8/20/2019
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