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Catalog Number Size Price
LS-C661947-10 10 µg $318 
LS-C661947-100 100 µg $470 
MMP2 Antibody - Western blot analysis of MMP2 using anti-MMP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human MMP2 protein 1ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP2 at approximately 71KD. The expected band size for MMP2 is at 71KD.

Polyclonal Rabbit anti‑Human MMP2 Antibody (WB) LS‑C661947

Polyclonal Rabbit anti‑Human MMP2 Antibody (WB) LS‑C661947

Antibody:
MMP2 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661947-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MMP2 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MMP2 antibody LS-C661947 is an unconjugated rabbit polyclonal antibody to human MMP2. Validated for ELISA and WB.
Target
Human MMP2
Synonyms
MMP2 | 72 kd type iv collagenase | 72 kDa gelatinase | 72 kd gelatinase | 72 kDa type IV collagenase | CLG4A | CLG4 | GelA | Gelatinase A | Matrix metalloproteinase-2 | MMP-2 | MONA | Neutrophil gelatinase | TBE-1 | Tbe-1 metalloprotease | Matrix metalloproteinase-II | MMP-II
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively.
Specificity
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. .
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MMP2
P08253 NM_004530 NP_004521.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/2/2024