JNK1 antibody LS-C17754 is an unconjugated rabbit polyclonal antibody to JNK1 (MAPK8) from human, mouse and rat. Validated for ELISA, IHC, IP and WB.
Human MAPK8 / JNK1
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:75)
- Western blot (1:1000)
- Immunoprecipitation (1:200)
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MAPK8 / JNK1 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK.
Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species cross-reactivity: mouse, monkey, hamster, bovine, rat and Drosophila. Species sequence homology: Xenopus.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:200. Immunohistochemistry (Paraffin): 1:100.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.