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MAPK8 / JNK1 Antibody (phospho‑Thr183/Tyr185/Thr221/Tyr223) LS‑C16104

Antibody

MAPK8 / JNK1 Antibody (phospho‑Thr183/Tyr185/Thr221/Tyr223) LS‑C16104

Antibody:
Rabbit Polyclonal to Human MAPK8 / JNK1
Application:
WB, IP
Reactivity:
Human, Mouse, Rat, Chicken, Drosophila, C. elegans
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$605 
LS-C16104-200
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MAPK8 / JNK1
Application:
WB, IP
Reactivity:
Human, Mouse, Rat, Chicken, Drosophila, C. elegans
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
JNK1 antibody LS-C16104 is an unconjugated rabbit polyclonal antibody to JNK1 (MAPK8) from human, mouse, rat and other species. Validated for IP and WB.
Target
Human MAPK8 / JNK1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Drosophila, C. elegans (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • Immunoprecipitation (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MAPK8 / JNK1 antibody was raised against synthetic peptide corresponding to aa181-190 [(MM[pT]P[pY]VVTRY-C)] of human/mouse/rat JNK1 (MAPK 8) and human/mouse/rat JNK2 (MAPK 9). Sequence also matches aa219-229 of human/mouse/rat JNK3.
Epitope
pThr183/Tyr185/Thr221/Tyr223
Specificity
Recognizes phosphorylated JNK, Mr 46kD and 55kD. Species cross-reactivity: Human, rat, mouse. Expected to cross-react with Drosophila, C. elegans and chicken based on sequence homology.
Usage
Suitable for use in Immunoprecipitation/Western Blot. Immunoprecipitation/Western Blot: 1 ug/ml. Anti-pan JNK/SAPK1 was used to Immunoprecipitate JNK from lysates of ultraviolet light-treated RAW 264 macrophage. Phosphorylated JNK (T183/Y185) was detected. Phosphorylated JNK was not detected in non-treated lysates.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK8 / JNK1
P45983 NM_139049 NP_620637.1

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: IHC - Paraffin, Western blot
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: All species
Range: 15.6-1000 pg/ml

Requested From: United States
Date Requested: 7/22/2019
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