Target
Human MAPK8 / JNK1
Synonyms
MAPK8 | JNK | JNK1 | JNK1A2 | JNK-46 | MAP kinase 8 | PRKM8 | SAPK1 | SAPK1c | MAPK 8 | C-Jun N-terminal kinase 1 | JNK21B1/2 | JUN N-terminal kinase
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK (KLH coupled).
Specificity
Detects endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (1:2000)
- Immunoprecipitation (1:250)
- Flow Cytometry (1:200)
- ELISA
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Flow Cytometry. Immunoprecipitation: 1:250. Flow Cytometry: 1:200.
Presentation
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee