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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPK14 / p38 Antibody for IHC, IF/Immunofluorescence, ELISA LS-C502279

P38 antibody LS-C502279 is an unconjugated rabbit polyclonal antibody to human p38 (MAPK14). Validated for ELISA, IF and IHC.
Catalog
Size
Price
LS-C502279-50
50 µg
$285
LS-C502279-100
100 µg
$385
Description
P38 antibody LS-C502279 is an unconjugated rabbit polyclonal antibody to human p38 (MAPK14). Validated for ELISA, IF and IHC.
Target
Human MAPK14 / p38
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MAPK14 / p38 antibody was raised against recombinant Human Mitogen-activated protein kinase 14 protein (2-258AA)
Specificity
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MAPK14 / p38
Q16539 NM_001315 NP_001306.1

Popular MAPK14 / p38 Products

Western blot of C-6 glioma cell lysates in the presence of Anisomycin showing specific immunolabeling of the ~39k p38 protein phosphorylated at Thr180 and Tyr182 (Anisomycin). The immunolabeling is absent without Anisomycin (Control).
Species: Rat, Human, Mouse, Bovine, Dog, Chicken
Applications: IHC, Western blot
MAPK14 Antibody (Y322) western blot of Jurkat cell line and mouse kidney tissue lysates (35 ug/lane). The MAPK14 antibody detected the MAPK14 protein (arrow).
Species: Human
Applications: Western blot, Flow Cytometry
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p38 MAPK (Phospho-Tyr182) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-MAPK14 / p38 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Mouse, Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
Western blot of MAPK14 / P38 antibody.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation

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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using MAPK14 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using MAPK14 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Requested From: United States
Date Requested: 1/20/2019
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