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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPK1 / ERK2 Antibody LS‑C16340

Antibody

MAPK1 / ERK2 Antibody LS‑C16340

Antibody:
Mouse Monoclonal to Rat MAPK1 / ERK2
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$530 
LS-C16340-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Rat MAPK1 / ERK2
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ERK2 antibody LS-C16340 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) from mouse and rat. Validated for ELISA, IHC and WB.
Target
Rat MAPK1 / ERK2
Host
Mouse
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MAPK1 / ERK2 antibody was raised against synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Specificity
Detects endogenous levels of total p42 MAP kinase (Erk2) protein. Recognizes p44 MAP kinase (Erk1) in some cell types, although with lower affinity. It does not cross-react with either JNK/ SAPK or p38 MAP kinase. Species cross-reactivity: Human and mouse.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:2000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK1 / ERK2
P28482 NM_002745 NP_002736.3

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Requested From: United States
Date Requested: 7/22/2019
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