ERK2 antibody LS-C16340 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) from mouse and rat. Validated for ELISA, IHC and WB.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- Western blot (1:2000)
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MAPK1 / ERK2 antibody was raised against synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Detects endogenous levels of total p42 MAP kinase (Erk2) protein. Recognizes p44 MAP kinase (Erk1) in some cell types, although with lower affinity. It does not cross-react with either JNK/ SAPK or p38 MAP kinase. Species cross-reactivity: Human and mouse.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:2000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.