ERK2 antibody LS-C16375 is an unconjugated rabbit polyclonal antibody to ERK2 (MAPK1) from human, mouse and rat. Validated for IP and WB.
Mouse MAPK1 / ERK2
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
MAPK1 / ERK2 antibody was raised against recombinant mouse ERK2.
Recognizes the 42kD MAP Kinase encoded by the mapk gene of Human and rat.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 3T3 cell lysate. Use 20 ug per lane for minigels. Applications: Suitable for use in Western Blot, Immunoprecipitation. Recommended Dilution: Western Blot: 0.5-2 ug/ml detected p42MAP Kinase (erk2) in human A431 carcinoma cells, mouse 3T3 fibroblasts and rat L6 skeletal fibroblasts. Immunoprecipitation: 5 ug of this antibody immunoprecipitated p42MAPK (erk2) from 3T3 cells as confirmed by Western Western Blot using the monoclonal anti-erk2.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.