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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPK1 / ERK2 Antibody LS-C16323

ERK2 antibody LS-C16323 is an unconjugated rabbit polyclonal antibody to ERK2 (MAPK1) from human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Catalog
Size
Price
LS-C16323-200
200 µl
$530
Description
ERK2 antibody LS-C16323 is an unconjugated rabbit polyclonal antibody to ERK2 (MAPK1) from human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human MAPK1 / ERK2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Agarose.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:1000)
  • Flow Cytometry (1:25)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MAPK1 / ERK2 antibody was raised against synthetic peptide (KLH coupled) derived from the sequence of human p38 MAP kinase.
Specificity
Detects endogenous levels of total p38 MAP kinase protein. Does not recognize either JNK/SAPK or p44/42 MAP kinase. Species cross-reactivity: human, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry (Paraffin sections) and Flow Cytometry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Flow Cytometry: 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MAPK1 / ERK2
P28482 NM_002745 NP_002736.3

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Requested From: United States
Date Requested: 4/21/2019
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