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Catalog Number Size Price
LS-C750585-20 20 µl $253 
LS-C750585-50 50 µl $306 
LS-C750585-100 100 µl $381 
LS-C750585-200 200 µl $489 
LMNA / Lamin A+C Antibody - Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody at 1:2000 dilution or LMNA antibody. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.
LMNA / Lamin A+C Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb. Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours.
LMNA / Lamin A+C Antibody - Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody at 1:2000 dilution or LMNA antibody. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.
LMNA / Lamin A+C Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb. Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours.
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Polyclonal Rabbit anti‑Human LMNA / Lamin A+C Antibody (phospho‑Ser22, WB) LS‑C750585

Polyclonal Rabbit anti‑Human LMNA / Lamin A+C Antibody (phospho‑Ser22, WB) LS‑C750585

Antibody:
LMNA / Lamin A+C Rabbit anti-Human Polyclonal (pSer22) Antibody
Application:
WB, IP
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C750585-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
LMNA / Lamin A+C Rabbit anti-Human Polyclonal (pSer22) Antibody
Application:
WB, IP
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Lamin A+C antibody LS-C750585 is an unconjugated rabbit polyclonal antibody to Lamin A+C (LMNA) (pSer22) from human. It is reactive with human and rat. Validated for IP and WB.
Target
Human LMNA / Lamin A+C
Synonyms
LMNA | 70 kDa lamin | CDCD1 | CMD1A | EMD2 | FPLD | IDC | FPL | FPLD2 | Lamin A/C | Lamin | LFP | LMNC | LMN1 | LMNL1 | HGPS | Prelamin-A/C | PRO1 | Lamin A | Lamin A/C-like 1 | LGMD1B | CDDC | CMT2B1 | LDP1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer22
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 62-74kDa, while the observed MW by Western blot was 69kDa, 78kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LMNA / Lamin A+C
P02545 NM_005572 NP_005563.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021