Lamin A+C antibody LS-B2973 is an unconjugated mouse monoclonal antibody to human Lamin A+C (LMNA). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human LMNA / Lamin A+C
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin (1:100)
- Western blot
- Immunoprecipitation (1:1)
- Flow Cytometry
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Recombinant human Lamin A.
The antibody reacts with both recombinant and native Lamin A and C in humans. It has been shown to bind to an epitope between amino acids 464-572
Immunohistochemistry: LS-B2973 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2973 was determined to be 1:100.
Tissue culture supernatant, 0.2 M Tris/HCl, pH 7.4, 5-10% fetal calf serum. Sourced in TCS.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.