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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIM Protein / LPP Antibody (aa32‑66) LS‑C393013
LPP antibody LS-C393013 is an unconjugated rabbit polyclonal antibody to LPP (LIM Protein) from human and mouse. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C393013-50
50 µl
$205

Popular LIM Protein / LPP Products

IHC using LPP Antibody (8B3A11)
Species: Human, Mouse, Hamster, Primate
Applications: IHC, ICC, Immunofluorescence, Western blot
Western blot of LPP monoclonal antibody against truncated LPP-His recombinant protein and HeLa cell lysate.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Detection of Human LPP by Immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-LPP used at a dilution of 1:200 (1 Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunocytochemical staining of a rat cardiac fibroblast using anti-Lipoma Preferred Partner, pAb (IG-817) (LPP; red fluorescence) and a green-fluorescent phalloidin derivative staining filamentous actin. LPP is a protein related to the cytoskeletal and focal adhesion protein zyxin.
Species: Human, Rat, Pig
Applications: IHC, ICC, Western blot, Immunoprecipitation

Product Description

LPP antibody LS-C393013 is an unconjugated rabbit polyclonal antibody to LPP (LIM Protein) from human and mouse. Validated for Peptide-ELISA and WB.
About LIM Protein / LPP
May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Q93052 NM_005578 NP_005569.1

LPP Antibody, LIM protein Antibody, Lipoma-preferred partner Antibody


Specifications

Target
Human LIM Protein / LPP
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Epitope
aa32-66
Specificity
This LPP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-66 amino acids from the human LPP.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Western blot

All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LPP Antibody (N-Term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Caki-1 whole cell lysate. Lane 4: HeLa whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MDA-MB-468 whole cell lysate. Lane 7: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Requested From: United States
Date Requested: 9/22/2018

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