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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIM Protein / LPP Antibody (aa107‑156, FITC) LS‑C457872
LPP antibody LS-C457872 is an FITC-conjugated rabbit polyclonal antibody to LPP (LIM Protein) from human, mouse, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C457872-100
100 µl (1 mg/ml)
$385
Description
LPP antibody LS-C457872 is an FITC-conjugated rabbit polyclonal antibody to LPP (LIM Protein) from human, mouse, bovine and other species. Validated for WB.
Target
Human LIM Protein / LPP
Host
Rabbit
Reactivity
Human, Chimpanzee, Gibbon, Mouse, Bovine (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Dog, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
FITC. Also available Unconjugated or conjugated with Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa107-156 of human LPP (Q93052, NP_005569). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse (100%); Galago, Marmoset, Panda, Dog, Rabbit, Horse (92%); Bat (91%); Elephant, Bovine (81%).
Epitope
aa107-156
Specificity
Human LPP
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About LIM Protein / LPP
Q93052 NM_005578 NP_005569.1

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Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Applications: IHC, IHC - Paraffin, Western blot, ELISA

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Images

Western blot

LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
LPP antibody Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/12/2018
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