Human LF / LTF / Lactoferrin

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated or conjugated with FITC, HRP.

Ion exchange chromatography

Unmodified

Human Lactoferrin.

Human LTF / Lactoferrin. In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. Cross-reactivity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.

• IHC
• Western blot
• ELISA
• Immunodiffusion
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. This product is intended for use in immunocytochemical and immunohistochemical techniques for the detection of human lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000.

Lyophilized from PBS, pH 7.2

Use 1 ml of sterile Distilled water.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02788 NCBI: NM_002343 NP_002334.2