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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Lewis A Blood Group Antigen Antibody (clone SPM279, Azide‑free) LS‑C391433
Lewis A Blood Group Antigen antibody LS-C391433 is an unconjugated mouse monoclonal antibody to Lewis A Blood Group Antigen from human and mouse. Validated for Flow, IF and IHC.
Catalog
Size
Price
LS-C391433-100
100 µg (1 mg/ml)
$415

Popular Lewis A Blood Group Antigen Products

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ELISA
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (7LE).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (7LE).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry

Product Description

Lewis A Blood Group Antigen Antibody (clone SPM279, Azide-free) for IHC, IF/Immunofluorescence, Flow LS-C391433

Specifications

Target
Human Lewis A Blood Group Antigen
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [SPM279]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified or Ready-to-Use.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Mucins isolated from ovarian cyst fluid were used as the immunogen for the Blood Group Lewis a antibody.
Specificity
Human Blood Group Antigen Lewis A
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a carbohydrate determinant of Gal 1-3 (Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. Optimal dilution of the Blood Group Lewis a antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/16/2018

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