Human Lewis A Blood Group Antigen
(tested or 100% immunogen sequence identity)
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
- IHC - Paraffin
- Western blot
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Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
The antibody 7LE recognizes Lewis<sup>a</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis<sup>a</sup> may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
Immunohistochemistry (paraffin sections): Recommended dilution - 5 ug/ml. Positive control - colorectal adenocarcinoma. Application note - heat mediated antigen retrieval (sodium citrate). mAb incubation 1 hour / RT. detection DAB.
PBS, pH 7.4, 15 mM Sodium Azide
Store at 2°C to 8°C. Do not freeze.
For research use only.