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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Lewis A Blood Group Antigen Antibody (clone 7LE) LS‑C46047 Best Seller
Lewis A Blood Group Antigen antibody LS-C46047 is an unconjugated mouse monoclonal antibody to human Lewis A Blood Group Antigen. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C46047-100
100 µg (1 mg/ml)
$240
Description
Lewis A Blood Group Antigen antibody LS-C46047 is an unconjugated mouse monoclonal antibody to human Lewis A Blood Group Antigen. Validated for ELISA, IHC and WB.
Target
Human Lewis A Blood Group Antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [7LE]
Conjugations
Unconjugated
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
Specificity
The antibody 7LE recognizes Lewis<sup>a</sup> blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis<sup>a</sup> may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
Usage
Immunohistochemistry (paraffin sections): Recommended dilution - 5 ug/ml. Positive control - colorectal adenocarcinoma. Application note - heat mediated antigen retrieval (sodium citrate). mAb incubation 1 hour / RT. detection DAB.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.

Popular Lewis A Blood Group Antigen Products

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ELISA
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (7LE).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (7LE).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry

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Images

Immunohistochemistry

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.
Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.

Immunohistochemistry

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.
Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.

Immunohistochemistry

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.
Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.

Immunohistochemistry

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.
Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). Commercially tested by LifeSpan BioSciences.

Requested From: United States
Date Requested: 12/18/2018
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