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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Leptin Antibody LS-C355514

Note: This antibody replaces LS-C25175
Leptin antibody LS-C355514 is an unconjugated rabbit polyclonal antibody to Leptin from human, mouse, bat and other species. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C355514-50
50 µl
$445
Description
Leptin antibody LS-C355514 is an unconjugated rabbit polyclonal antibody to Leptin from human, mouse, bat and other species. Validated for ICC, IHC and WB.
Target
Human Leptin
Host
Rabbit
Reactivity
Human, Chimpanzee, Gibbon, Mouse, Bat, Hamster (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Rat, Bovine, Goat, Horse, Sheep (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Western blot (1:200 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Leptin antibody was raised against synthetic peptide corresponding to aa25-44 (QKVQDDTKTLIKTIVTRIND) of mouse Leptin. Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Mouse, Bat, Hamster (100%); Gorilla, Rat, Sheep, Bovine, Goat, Panda, Horse, Zebu (95%); Orangutan (90%).
Specificity
The antibody detects leptin from human, mouse, sheep and rat samples. It has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kD protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with It results in staining of the ventromedial hypothalamus. This sequence is completely conserved between mouse and bovine.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About Leptin
P41159 NM_000230 NP_000221.1

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Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Immunohistochemistry

IHC using Leptin Antibody
IHC using Leptin Antibody

Western blot

WB using Leptin Antibody
WB using Leptin Antibody

Immunohistochemistry

IHC using Leptin Antibody
IHC using Leptin Antibody

Western blot

WB using Leptin Antibody
WB using Leptin Antibody

Immunohistochemistry

IHC using Leptin Antibody
IHC using Leptin Antibody

Western blot

WB using Leptin Antibody
WB using Leptin Antibody

Immunohistochemistry

IHC using Leptin Antibody
IHC using Leptin Antibody

Western blot

WB using Leptin Antibody
WB using Leptin Antibody

Requested From: United States
Date Requested: 4/18/2019
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