Human LCAT protein amino acids 132-143 (SVEYLDSSKLAG). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Marmoset, Sheep, Bovine (100%); Horse, Pig, Guinea pig, Chicken, Platypus, Xenopus (92%); Rat, Dog, Panda, Rabbit, Opossum (83%).
Expected to react with many animal sources.
Plasma samples should be diluted prior to electrophoresis and immunoblotting to avoid the band-broadening effect caused by comigration with endogenous albumin.
TBS, pH 7.4, containing 50% glycerol and 0.02% sodium azide
Store at -20°C, stable for 1 year. Avoid freeze-thaw cycles.
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