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LCAT

lecithin-cholesterol acyltransferase

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.

Gene Name: lecithin-cholesterol acyltransferase
Synonyms: LCAT
Target Sequences: NM_000229 NP_000220.1 P04180

Publications (8)

1
Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase. Chisholm JW, Gebre AK, Parks JS. Journal of lipid research. 1999 40:1512-9. [PubMed:10428989]
2
Amino acids 149 and 294 of human lecithin:cholesterol acyltransferase affect fatty acyl specificity. Zhao Y, Gebre AK, Parks JS. Journal of lipid research. 2004 45:2310-6. (ELISA; Mouse) [PubMed:15375182]
3
Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice. Lee JY, Badeau RM, Mulya A, Boudyguina E, Gebre AK, Smith TL, Parks JS. Journal of lipid research. 2007 48:1052-61. [PubMed:17272829]
4
Control of cholesteryl ester transfer protein activity by sequestration of lipid transfer inhibitor protein in an inactive complex. He Y, Greene DJ, Kinter M, Morton RE. Journal of lipid research. 2008 49:1529-37. (WB; Human) [PubMed:18369235] [PMC:PMC2431105]
5
Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL. Mulya A, Lee JY, Gebre AK, Boudyguina EY, Chung SK, Smith TL, Colvin PL, Jiang XC, Parks JS. Journal of lipid research. 2008 49:2390-401. [PubMed:18583707] [PMC:PMC2563212]
6
LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins. Hirsch-Reinshagen V, Donkin J, Stukas S, Chan J, Wilkinson A, Fan J, Parks JS, Kuivenhoven JA, Ltjohann D, Pritchard H, Wellington CL. Journal of lipid research. 2009 50:885-93. [PubMed:19065001] [PMC:PMC2666175]
7
Lecithin: cholesterol acyltransferase expression has minimal effects on macrophage reverse cholesterol transport in vivo. Tanigawa H, Billheimer JT, Tohyama J, Fuki IV, Ng DS, Rothblat GH, Rader DJ. Circulation. 2009 120:160-9. (WB; Human) [PubMed:19564558] [PMC:PMC2796275]
8
The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Bogan RL, Hennebold JD. Reproduction (Cambridge, England). 2010 139:163-76. (IHC) [PubMed:19776099] [PMC:PMC3255460]
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Antibodies (95)
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LCAT (95)
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monoclonal mc (9)
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aa41-210 (24)
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aa362-411 (4)
C-Terminus (3)
aa300-313 (1)
aa389-423 (1)
No (95)
LCAT Antibody - Immunofluorescence of monoclonal antibody to LCAT on HeLa cell . [antibody concentration 10 ug/ml]
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LCAT Mouse anti-Human Monoclonal (4A9) Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$423
LCAT Antibody - Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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LCAT Rabbit anti-Human Polyclonal (aa362-411) Antibody
Human
WB
Unconjugated
100 µl/$424
LCAT Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
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LCAT Goat anti-Human Polyclonal Antibody
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$460
LCAT Antibody - Western blot analysis of LCAT using HuvEc whole cells lysates
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LCAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
LCAT Antibody - LCAT Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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LCAT Rabbit anti-Human Polyclonal (aa285-313) Antibody
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400 µl/$393
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LCAT Rabbit anti-Human Polyclonal (aa285-313) Antibody
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
200 µl/$734
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LCAT Rabbit anti-Human Polyclonal (aa285-313) (Biotin) Antibody
Human
ELISA, Flo, ICC, IF, IHC, WB
Biotin Conjugated
200 µl/$952
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LCAT Rabbit anti-Human Polyclonal (aa285-313) (FITC) Antibody
Human
ELISA, Flo, ICC, IF, IHC, WB
FITC Conjugated
200 µl/$952
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LCAT Rabbit anti-Human Polyclonal (aa285-313) (APC) Antibody
Human
ELISA, Flo, ICC, IF, IHC, WB
APC Conjugated
200 µl/$952
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LCAT Rabbit anti-Human Polyclonal (aa285-313) (Azide-free) (HRP) Antibody
Human
ELISA, Flo, ICC, IF, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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LCAT Rabbit anti-Human Polyclonal (aa285-313) (PE) Antibody
Human
ELISA, Flo, ICC, IF, IHC, WB
PE Conjugated
200 µl/$952
LCAT Antibody - Anti-LCAT antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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LCAT Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
LCAT Antibody - Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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LCAT Rabbit anti-Human Polyclonal (aa290-433) Antibody
Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
LCAT Antibody - Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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LCAT Rabbit anti-Human Polyclonal (aa290-433) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$319; 200 µl/$365; 500 µl/$519; 1 ml/$596; 2 ml/$824; 5 ml/$1,348
LCAT Antibody - Western Blot; Sample: Recombinant LCAT, Human.
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LCAT Rabbit anti-Human Polyclonal (aa41-210) Antibody
Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
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LCAT Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$341
LCAT Antibody - Immunochemical staining of human LCAT in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
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LCAT Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ELISA, IHC-P
Unconjugated, Preservative Free
100 µl/$412
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LCAT Rabbit anti-Mouse Polyclonal Antibody
Mouse
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
LCAT Antibody - Goat Anti-LCAT Antibody (1µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
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LCAT Goat anti-Human Polyclonal Antibody
Human
Peptide-ELISA, WB
Unconjugated
100 µg/$503
LCAT Antibody - Immunohistochemistry of paraffin-embedded Rat brain using LCAT Polyclonal Antibody at dilution of 1:100 (40x lens).
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LCAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
LCAT Antibody - LCAT Antibody - Western blot of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot of rabbit antiserum against mouse LCAT.
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LCAT Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Mouse, Human
WB
Unconjugated
0.1 ml/$386
LCAT Antibody - Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
LCAT Rabbit anti-Mouse Polyclonal (aa41-210) Antibody
Mouse
WB
Unconjugated
100 µl/$311; 200 µl/$355; 500 µl/$499; 1 ml/$571; 2 ml/$777; 5 ml/$1,261
LCAT Antibody - LCAT antibody Western blot. All lanes: Anti LCAT at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: HEPG2 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.
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LCAT Rabbit anti-Mouse Polyclonal (aa389-423) Antibody
Mouse, Rat, Human
WB
Unconjugated
10 µg/$318; 100 µg/$470
LCAT Antibody - Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
LCAT Rabbit anti-Mouse Polyclonal (aa290-432) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$311; 200 µl/$355; 500 µl/$499; 1 ml/$571; 2 ml/$777; 5 ml/$1,261
LCAT Antibody - Western blot of recombinant LCAT.
Select
LCAT Mouse anti-Human Monoclonal (aa290-433) Antibody
Human
WB
Unconjugated
100 µl/$314; 200 µl/$359; 500 µl/$507; 1 ml/$581; 2 ml/$798; 5 ml/$1,296
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).