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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LCAT

lecithin-cholesterol acyltransferase

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.

Gene Name: lecithin-cholesterol acyltransferase
Synonyms: LCAT
Target Sequences: NM_000229 NP_000220.1 P04180

Publications (8)

1
Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase. Chisholm JW, Gebre AK, Parks JS. Journal of lipid research. 1999 40:1512-9. [PubMed:10428989]
2
Amino acids 149 and 294 of human lecithin:cholesterol acyltransferase affect fatty acyl specificity. Zhao Y, Gebre AK, Parks JS. Journal of lipid research. 2004 45:2310-6. (ELISA; Mouse) [PubMed:15375182]
3
Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice. Lee JY, Badeau RM, Mulya A, Boudyguina E, Gebre AK, Smith TL, Parks JS. Journal of lipid research. 2007 48:1052-61. [PubMed:17272829]
4
Control of cholesteryl ester transfer protein activity by sequestration of lipid transfer inhibitor protein in an inactive complex. He Y, Greene DJ, Kinter M, Morton RE. Journal of lipid research. 2008 49:1529-37. (WB; Human) [PubMed:18369235] [PMC:PMC2431105]
5
Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL. Mulya A, Lee JY, Gebre AK, Boudyguina EY, Chung SK, Smith TL, Colvin PL, Jiang XC, Parks JS. Journal of lipid research. 2008 49:2390-401. [PubMed:18583707] [PMC:PMC2563212]
6
LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins. Hirsch-Reinshagen V, Donkin J, Stukas S, Chan J, Wilkinson A, Fan J, Parks JS, Kuivenhoven JA, Ltjohann D, Pritchard H, Wellington CL. Journal of lipid research. 2009 50:885-93. [PubMed:19065001] [PMC:PMC2666175]
7
Lecithin: cholesterol acyltransferase expression has minimal effects on macrophage reverse cholesterol transport in vivo. Tanigawa H, Billheimer JT, Tohyama J, Fuki IV, Ng DS, Rothblat GH, Rader DJ. Circulation. 2009 120:160-9. (WB; Human) [PubMed:19564558] [PMC:PMC2796275]
8
The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Bogan RL, Hennebold JD. Reproduction (Cambridge, England). 2010 139:163-76. (IHC) [PubMed:19776099] [PMC:PMC3255460]
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Filtered by: None
Target
LCAT (95)
Reactivity
Human (70)
Mouse (31)
Rat (28)
Bovine (9)
Chimpanzee (6)
Dog (8)
Gibbon (6)
Guinea pig (8)
Hamster (4)
Horse (8)
Monkey (10)
Pig (8)
Rabbit (8)
Sheep (9)
Application
IHC (10)
IHC-P (3)
WB (95)
Flo (8)
ELISA (10)
ICC (6)
IF (9)
Peptide-ELISA (2)
Host
rabbit (84)
mouse (9)
goat (2)
Isotype
IgG (38)
IgG2a,k (1)
Clonality
monoclonal mc (9)
polyclonal pc (86)
Clone
4A9 (1)
Format
AP Conjugated (2)
APC (7)
APC Conjugated (9)
Azide-free (1)
Biotin Conjugated (11)
Cy3 Conjugated (7)
Cy7 Conjugated (7)
FITC Conjugated (11)
HRP Conjugated (11)
PE Conjugated (9)
Unconjugated (28)
Epitope
aa41-210 (24)
aa290-433 (16)
aa285-313 (8)
aa290-432 (8)
aa290-434 (8)
N-Terminus (4)
aa362-411 (4)
C-Terminus (3)
aa300-313 (1)
aa389-423 (1)
Publications
No (95)
Showing 1-25 of 95 results

IHC-plus™ LCAT Antibody LS-B15610
Goat Polyclonal to Human LCAT
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µg  $425.00
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
Goat Anti-LCAT (aa366-378) Antibody (1µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

LCAT Antibody (clone 4A9) LS-C197605
Mouse Monoclonal [clone 4A9] (IgG2a,k) to Human LCAT
Human
ELISA, IF, WB
Unconjugated
100µg  $365.00
Immunofluorescence of monoclonal antibody to LCAT on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of LCAT expression in transfected 293T cell line by LCAT monoclonal antibody (M01), clone 4A9.Lane 1: LCAT transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.

LCAT Antibody (aa362-411) LS-C80450
Rabbit Polyclonal (IgG) to Human LCAT
Rabbit, Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

LCAT Antibody (aa290-433) LS-C295100
Rabbit Polyclonal to Human LCAT
Human
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00
Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

LCAT Antibody (aa290-433, Biotin) LS-C298061
Rabbit Polyclonal to Human LCAT
Human
WB
Biotin Conjugated
100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $575.00
Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

LCAT Antibody (aa285-313) LS-C165713
Rabbit Polyclonal to Human LCAT
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
LCAT Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Confocal immunofluorescence of LCAT Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

LCAT Antibody (aa285-313) LS-C322608
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
200µl  $590.00

LCAT Antibody (aa285-313, Biotin) LS-C261749
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, ICC, IF, IHC, WB
Biotin Conjugated
200µl  $740.00

LCAT Antibody (aa285-313, FITC) LS-C261751
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, ICC, IF, IHC, WB
FITC Conjugated
200µl  $740.00

LCAT Antibody (aa285-313, PE) LS-C261754
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, ICC, IF, IHC, WB
PE Conjugated
200µl  $740.00

LCAT Antibody (aa285-313, Azide-free, HRP) LS-C261753
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, ICC, IF, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

LCAT Antibody (aa285-313, AP) LS-C261748
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, ICC, IF, IHC, WB
AP Conjugated
200µl  $740.00

LCAT Antibody (aa285-313, APC) LS-C261747
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, Flo, ICC, IF, IHC, WB
APC Conjugated
200µl  $740.00

IHC-plus™ LCAT Antibody (C-Terminus) LS-B252
Rabbit Polyclonal to Human LCAT
Human
IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Anti-LCAT antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LCAT Antibody (aa41-210) LS-C374943
Rabbit Polyclonal to Human LCAT
Human
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00
Western Blot; Sample: Recombinant LCAT, Human.

LCAT Antibody LS-C752685
Rabbit Polyclonal (IgG) to Human LCAT
Human
ELISA, WB
Unconjugated
100µg  $355.00

LCAT Antibody LS-C807037
Rabbit Polyclonal (IgG) to Human LCAT
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Western blot Image

LCAT Antibody LS-C502454
Goat Polyclonal to Human LCAT
Human
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Goat Anti-LCAT Antibody (1µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

LCAT Antibody LS-C767849
Rabbit Polyclonal (IgG) to Mouse LCAT
Mouse
WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

LCAT Antibody (C-Terminus) LS-C142502
Rabbit Polyclonal to Mouse LCAT
Mouse, Human
WB
Unconjugated
100µl  $335.00
LCAT Antibody - Western blot of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot of rabbit antiserum against mouse LCAT.

LCAT Antibody (aa41-210) LS-C295102
Rabbit Polyclonal to Mouse LCAT
Mouse
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00
Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

LCAT Antibody (aa389-423) LS-C407869
Rabbit Polyclonal to Mouse LCAT
Mouse, Rat, Human
WB
Unconjugated
100µg  $325.00
LCAT antibody Western blot. All lanes: Anti LCAT at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: HEPG2 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.

LCAT Antibody (aa290-432) LS-C295104
Rabbit Polyclonal to Mouse LCAT
Mouse, Human
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00
Western blot of recombinant LCAT.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot; Sample: Mouse Serum; Primary Ab: 2µg/ml Rabbit Anti-Mouse LCAT Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

LCAT Antibody (aa290-433) LS-C314323
Mouse Monoclonal (IgG) to Human LCAT
Human
WB
Unconjugated
20µl 1mg/ml  $205.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $305.00; 1ml 1mg/ml  $560.00
Western blot of recombinant LCAT.
Western Blot; Sample: Recombinant LCAT, Human.

LCAT Antibody LS-C311333
Rabbit Polyclonal (IgG) to Human LCAT
Human
WB
Unconjugated
100µg  $605.00
Showing 1-25 of 95 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).