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Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase. Chisholm JW, Gebre AK, Parks JS. Journal of lipid research. 1999 40:1512-9.
Amino acids 149 and 294 of human lecithin:cholesterol acyltransferase affect fatty acyl specificity. Zhao Y, Gebre AK, Parks JS. Journal of lipid research. 2004 45:2310-6. (ELISA; Mouse)
Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice. Lee JY, Badeau RM, Mulya A, Boudyguina E, Gebre AK, Smith TL, Parks JS. Journal of lipid research. 2007 48:1052-61.
Control of cholesteryl ester transfer protein activity by sequestration of lipid transfer inhibitor protein in an inactive complex. He Y, Greene DJ, Kinter M, Morton RE. Journal of lipid research. 2008 49:1529-37. (WB; Human)
Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL. Mulya A, Lee JY, Gebre AK, Boudyguina EY, Chung SK, Smith TL, Colvin PL, Jiang XC, Parks JS. Journal of lipid research. 2008 49:2390-401.
LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins. Hirsch-Reinshagen V, Donkin J, Stukas S, Chan J, Wilkinson A, Fan J, Parks JS, Kuivenhoven JA, Ltjohann D, Pritchard H, Wellington CL. Journal of lipid research. 2009 50:885-93.
Lecithin: cholesterol acyltransferase expression has minimal effects on macrophage reverse cholesterol transport in vivo. Tanigawa H, Billheimer JT, Tohyama J, Fuki IV, Ng DS, Rothblat GH, Rader DJ. Circulation. 2009 120:160-9. (WB; Human)
The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Bogan RL, Hennebold JD. Reproduction (Cambridge, England). 2010 139:163-76. (IHC)