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KLH Antibody (Biotin) LS‑C154295

Catalog Number Size Price
LS-C154295-100 100 µg (1 mg/ml) $425 
Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.

KLH Antibody (Biotin) LS‑C154295

Antibody:
Rabbit Polyclonal to Keyhole Limpet KLH
Application:
WB, ELISA
Reactivity:
Keyhole Limpet
Format:
Biotin, Unmodified
Price
Catalog Number
$425 
LS-C154295-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Keyhole Limpet KLH
Application:
WB, ELISA
Reactivity:
Keyhole Limpet
Format:
Biotin, Unmodified

Specifications

Description
KLH antibody LS-C154295 is a biotin-conjugated rabbit polyclonal antibody to keyhole limpet KLH. Validated for ELISA and WB.
Target
Keyhole Limpet KLH
Host
Rabbit
Reactivity
Keyhole Limpet (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:5000 - 1:25000)
  • ELISA
Immunogen
KLH (Keyhole Limpet Hemocyanin.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Biotin.
Usage
Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Anti-KLH antibody biotin conjugate is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Product
Species: Keyhole Limpet
Applications: Immunofluorescence, Western blot, Flow Cytometry
Product
Species: Keyhole Limpet
Applications: IHC, Western blot, ELISA
Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results.
Species: Keyhole Limpet
Applications: IHC, Western blot, ELISA
Product
Species: Keyhole Limpet
Applications: ELISA
Product
Species: Keyhole Limpet
Applications: ELISA
Product
Species: Keyhole Limpet
Applications: ELISA

Requested From: United States
Date Requested: 8/19/2019
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