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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
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KLH

KLH / Keyhole Limpet Hemocyanin

Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein that is found in the hemolymph of the giant keyhole limpet, Megathura crenulata, a species of keyhole limpet that lives off the coast of California, from Monterey Bay to Isla Asuncion off Baja California. KLH is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier protein for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high hapten:carrier protein ratio, increasing the likelihood of generating hapten-specific antibodies. In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms.

KLH Target Details

Target Name: KLH / Keyhole Limpet Hemocyanin
Target Abbreviation: KLH


Select
Mouse Monoclonal [clone 12B4.G3.A8] (IgG1,k) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
500µg   1mg/ml   $335.00
KLH Antibody - Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
1mg   1mg/ml   $495.00
Select
Rabbit Polyclonal to Keyhole Limpet KLH
Keyhole Limpet
Flo, IF, WB
FITC Conjugated
100µl   $740.00
Select
Mouse Monoclonal (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
1mg   $485.00
Select
Chicken Polyclonal (IgY) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
HRP Conjugated
500µg   $425.00
Select
Mouse Monoclonal [clone 3B1] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
100µg   1mg/ml   $425.00
Select
Rabbit Polyclonal to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Biotin Conjugated
100µg   $740.00
Select
Chicken Polyclonal (IgY) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
500µg   $415.00
KLH Antibody - Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Biotin Conjugated
100µg   1mg/ml   $425.00
Select
Mouse Monoclonal [clone KT83] (IgG2a) to Keyhole Limpet KLH
Keyhole Limpet
Unconjugated
100µg   1mg/ml   $335.00
Select
Mouse Monoclonal [clone 3B1] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
25µl   1mg/ml   $235.00
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
HRP Conjugated
100µg   1mg/ml   $425.00
Select
Mouse Monoclonal [clone 6D12] (IgG1,k) to Megathura crenulata KLH
Megathura crenulata
ELISA, WB
Unconjugated
50µg   $285.00; 100µg   1mg/ml   $345.00
Select
Mouse Monoclonal [clone 6D12] (IgG1,k) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Unconjugated
100µl   $365.00
Select
Mouse Monoclonal to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Select
Mouse Monoclonal [clone 11E8F5C3] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Unconjugated, Preservative Free
20µg   $195.00; 100µg   $265.00; 200µg   $295.00; 500µg   $415.00
Select
Mouse Monoclonal [clone 11E8F5C3] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Biotin Conjugated, Preservative Free
50µg   $295.00
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
FLISA, IF, WB
TRITC Conjugated
100µl   1mg/ml   $425.00
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
FLISA, IF, WB
FITC Conjugated
100µl   1mg/ml   $425.00
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
FLISA, IF, WB
TR Conjugated
100µl   1mg/ml   $425.00
Select
Rabbit Polyclonal
ELISA, WB
Unconjugated
1ea   $345.00
Select
Mouse Monoclonal (IgG1,k) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
50µg   $365.00; 500µg   $495.00
Select
Mouse Monoclonal (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
1mg   $560.00
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
AP Conjugated
100µg   1mg/ml   $425.00
Select
Mouse Monoclonal (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
1mg   7.84mg/ml   $305.00
Viewing 1-25 of 51 products



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).