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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLH

KLH / Keyhole Limpet Hemocyanin

Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein that is found in the hemolymph of the giant keyhole limpet, Megathura crenulata, a species of keyhole limpet that lives off the coast of California, from Monterey Bay to Isla Asuncion off Baja California. KLH is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier protein for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high hapten:carrier protein ratio, increasing the likelihood of generating hapten-specific antibodies. In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms.

KLH Target Details

Target Name: KLH / Keyhole Limpet Hemocyanin
Target Abbreviation: KLH

Filtered by: None
Target
KLH (53)
Reactivity
Human (2)
Keyhole Limpet (43)
Megathura crenulata (1)
Application
IHC (6)
WB (15)
Flo (1)
DB (1)
ELISA (37)
IF (4)
Host
rabbit (13)
mouse (31)
chicken (9)
Isotype
IgG (9)
IgG1 (17)
IgG1,k (4)
IgG2a (1)
IgG2b (1)
IgG3 (1)
IgM (4)
IgY (4)
Clonality
monoclonal mc (31)
polyclonal pc (22)
Clone
11E8F5C3 (2)
12B4.G3.A8 (1)
3B1 (1)
3C1G5 (1)
6D12 (2)
Clone 1 (1)
KT82 (1)
KT83 (1)
KT84 (1)
KT85 (1)
Format
Agarose Conjugated (1)
AP Conjugated (3)
APC Conjugated (2)
Biotin Conjugated (7)
FITC Conjugated (6)
HRP Conjugated (5)
PE Conjugated (2)
Preservative Free (2)
TR Conjugated (1)
TRITC Conjugated (1)
Unconjugated (25)
Publications
No (53)
Showing 1-25 of 53 results

KLH Antibody (clone 12B4.G3.A8) LS-C154002
Mouse Monoclonal [clone 12B4.G3.A8] (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
500µg 1mg/ml  $335.00

KLH Antibody LS-C154226
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
1mg 1mg/ml  $495.00
Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results.

KLH Antibody (FITC) LS-C71310
Rabbit Polyclonal to Keyhole Limpet KLH
Keyhole Limpet
Flo, IF, WB
FITC Conjugated
100µl  $740.00

KLH Antibody LS-C71308
Mouse Monoclonal (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
1mg  $485.00

KLH Antibody (HRP) LS-C130347
Chicken Polyclonal (IgY) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
HRP Conjugated
500µg  $425.00

KLH Antibody (clone 3B1) LS-C153786
Mouse Monoclonal [clone 3B1] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
100µg 1mg/ml  $425.00

KLH Antibody (Biotin) LS-C71309
Rabbit Polyclonal to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
Biotin Conjugated
100µg  $740.00

KLH Antibody LS-C130340
Chicken Polyclonal (IgY) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
500µg  $415.00

KLH Antibody (Biotin) LS-C154295
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Biotin Conjugated
100µg 1mg/ml  $425.00
Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.

KLH Antibody (clone KT83) LS-C194707
Mouse Monoclonal [clone KT83] (IgG2a) to Keyhole Limpet KLH
Keyhole Limpet
Unconjugated
100µg  $345.00

KLH Antibody LS-C744846
Mouse Monoclonal (IgM)
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
25µl 1mg/ml  $235.00

KLH Antibody (HRP) LS-C154277
Rabbit Polyclonal (IgG) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, IHC, WB
HRP Conjugated
100µg 1mg/ml  $425.00

KLH Antibody (clone 11E8F5C3, Preservative Free) LS-C343407
Mouse Monoclonal [clone 11E8F5C3] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Unconjugated, Preservative Free
20µg  $195.00; 100µg  $265.00; 200µg  $295.00; 500µg  $415.00

KLH Antibody (clone 11E8F5C3, Preservative Free, Biotin) LS-C343291
Mouse Monoclonal [clone 11E8F5C3] (IgM) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Biotin Conjugated, Preservative Free
50µg  $295.00

KLH Antibody (clone 6D12) LS-C153322
Mouse Monoclonal [clone 6D12] (IgG1,k) to Megathura crenulata KLH
Megathura crenulata
ELISA, WB
Unconjugated
100µg 100µl 1mg/ml  $305.00

KLH Antibody (clone 6D12) LS-C144552
Mouse Monoclonal [clone 6D12] (IgG1,k) to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Unconjugated
100µl  $365.00

KLH Antibody LS-C487929
Mouse Monoclonal to Keyhole Limpet KLH
Keyhole Limpet
ELISA, WB
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

KLH Antibody LS-C186428
Rabbit Polyclonal
ELISA, WB
Unconjugated
1ea  $345.00

KLH Antibody LS-C51789
Rabbit Polyclonal
Keyhole Limpet
ELISA, WB
Unconjugated
200µg 1000µl 0.2mg/ml  $295.00

KLH Antibody (clone 3C1G5) LS-C742120
Mouse Monoclonal [clone 3C1G5] (IgG1,k)
DB, ELISA
Unconjugated
40µg  $245.00
Primary antibody: 1 µg/ml Mouse Anti-KLH Monoclonal Antibody Secondary antibody: IRDye TM 800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000, 610-132-121)

KLH Antibody LS-C194409
Mouse Monoclonal (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
1mg  $305.00

KLH Antibody LS-C130339
Mouse Monoclonal (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
1mg  $560.00

KLH Antibody LS-C189718
Mouse Monoclonal (IgG1,k) to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
50µg  $365.00; 500µg  $495.00

KLH Antibody LS-C487928
Rabbit Polyclonal to Keyhole Limpet KLH
Keyhole Limpet
ELISA
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

KLH Antibody (clone Clone 1) LS-C194582
Mouse Monoclonal [clone Clone 1] (IgG1) to Keyhole Limpet KLH
Keyhole Limpet
Unconjugated
1mg  $235.00
Showing 1-25 of 53 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).