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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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KLF4 Antibody (Ser457) LS-C98591

Fluorescent confocal image of HeLa cells stained KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.
Fluorescent confocal image of SY5Y cells stained KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.
Immunofluorescence of U251 cells, using KLF4 Antibody. Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Western blot of KLF4-S457 in MDA-MB231, NCI-H460 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.
Fluorescent confocal image of HeLa cells stained KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.
Fluorescent confocal image of SY5Y cells stained KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.
Immunofluorescence of U251 cells, using KLF4 Antibody. Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Western blot of KLF4-S457 in MDA-MB231, NCI-H460 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.
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KLF4 Antibody (Ser457) LS-C98591

Antibody:
Rabbit Polyclonal to Human KLF4
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C98591-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human KLF4
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
KLF4 antibody LS-C98591 is an unconjugated rabbit polyclonal antibody to human KLF4. Validated for IF and WB.

Target
Human KLF4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E7044 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98591. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Ser457
Specificity
This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-504 amino acids from human KLF4.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KLF4
O43474 NM_004235 NP_004226.3

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Featured Products

Product
Species: Human
Applications: Flow Cytometry
Reactivity: Human
Range: 31.2-2000 pg/ml
Reactivity: Rat
Range: 0.188-12 ng/ml
Reactivity: Mouse
Range: 31.25-2000 pg/ml
Product
Species: Mouse
Applications: IHC - Paraffin, IHC - Frozen, Western blot, ELISA

Requested From: United States
Date Requested: 5/25/2019
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