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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLF4 Antibody (aa11‑22) LS‑C55037
Note: This antibody replaces LS-C71405
KLF4 antibody LS-C55037 is an unconjugated goat polyclonal antibody to KLF4 from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55037-100
100 µg (0.5 mg/ml)
$405

Popular KLF4 Products

Anti-KLF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-KLF4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-KLF4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot

Product Description

KLF4 antibody LS-C55037 is an unconjugated goat polyclonal antibody to KLF4 from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.

Specifications

Target
Human KLF4
Synonyms
KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:64000) & (0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
KLF4 antibody was raised against synthetic peptide AVSDALLPSFST-C from the N-terminus of human KLF4 (NP_004226.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Cat, Dog, Bat, Horse, Rabbit, Pig (100%); Trichomonas (83%).
Blocking Peptide
LS-E25371 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55037. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa11-22
Specificity
Human KLF4.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About KLF4
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. O43474 NM_004235 NP_004226.3


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Images

Western blot

Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 11/14/2018

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