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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLF4 Antibody (clone 56CT5.1.6) LS‑C96469
KLF4 antibody LS-C96469 is an unconjugated mouse monoclonal antibody to KLF4 from human and mouse. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C96469-400
400 µl
$305
Description
KLF4 antibody LS-C96469 is an unconjugated mouse monoclonal antibody to KLF4 from human and mouse. Validated for Flow, IF and WB.
Target
Human KLF4
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [56CT5.1.6]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:200 - 1:2000)
  • Flow Cytometry (1:10 - 1:50)
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About KLF4
O43474 NM_004235 NP_004226.3

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Images

Immunofluorescence

Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.

Western blot

Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Flow Cytometry

Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Immunofluorescence

Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.

Western blot

Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Flow Cytometry

Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Immunofluorescence

Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.

Western blot

Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Flow Cytometry

Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Immunofluorescence

Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.

Western blot

Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Flow Cytometry

Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/21/2018
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