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Catalog Number Size Price
LS-C24764-100 100 µg $731 
Antibody

Monoclonal Mouse anti‑Chicken ITGA6/Integrin Alpha 6/CD49f Antibody (IHC, WB) LS‑C24764

Monoclonal Mouse anti‑Chicken ITGA6/Integrin Alpha 6/CD49f Antibody (IHC, WB) LS‑C24764

Antibody:
ITGA6/Integrin Alpha 6/CD49f Mouse anti-Chicken Monoclonal Antibody
Application:
IHC, ICC, WB, IP
Reactivity:
Chicken
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$731
LS-C24764-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ITGA6/Integrin Alpha 6/CD49f Mouse anti-Chicken Monoclonal Antibody
Application:
IHC, ICC, WB, IP
Reactivity:
Chicken
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD49f antibody LS-C24764 is an unconjugated mouse monoclonal antibody to chicken CD49f (ITGA6 / Integrin Alpha 6). Validated for ICC, IHC, IP and WB.
Target
Chicken ITGA6/Integrin Alpha 6/CD49f
Synonyms
ITGA6 | CD49f | CD49f antigen | Integrin alpha6B | Integrin, alpha 6 | Integrin alpha-6 precursor | VLA-6 | Integrin alpha-6 | ITGA6B
Host
Mouse
Reactivity
Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Purified
Modifications
Unmodified
Immunogen
Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6 M guanidine isothiocyanate, and depleted of beta1 subunit.
Specificity
Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.
Applications
  • IHC
  • ICC
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot. Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor. Immunohistochemistry: Methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 ug/ml, with overnight antibody incubation, followed by detection with a goat anti-mouse IgG-FITC secondary antibody. Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation). Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
Presentation
PBS, pH 7.6, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGA6/Integrin Alpha 6/CD49f
P23229 NM_000210 NP_000201.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/13/2022