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IHCPlus™ ITGA6/Integrin Alpha 6/CD49f Antibody aa1043‑1072 Polyclonal IF,IHC,WB LS‑B3626

Catalog Number Size Price
LS-B3626-200 200 µl $425 
Anti-ITGA6 / CD49f antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ITGA6 / CD49f antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).
Western blot of anti-ITGA6 Antibody (isoform 2 S1064) in 293 cell line lysates (35 ug/lane). ITGA6(arrow) was detected using the purified antibody.
Anti-ITGA6 / CD49f antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ITGA6 / CD49f antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).
Western blot of anti-ITGA6 Antibody (isoform 2 S1064) in 293 cell line lysates (35 ug/lane). ITGA6(arrow) was detected using the purified antibody.
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IHCPlus™ ITGA6/Integrin Alpha 6/CD49f Antibody aa1043‑1072 Polyclonal IF,IHC,WB LS‑B3626

Note: This antibody replaces LS-C98630
Antibody:
Rabbit Polyclonal to Human ITGA6/Integrin Alpha 6/CD49f
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3626-200
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ITGA6/Integrin Alpha 6/CD49f
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD49f antibody LS-B3626 is an unconjugated rabbit polyclonal antibody to human CD49f (ITGA6 / Integrin Alpha 6). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ITGA6/Integrin Alpha 6/CD49f
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7071 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3626. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1043-1072
Specificity
This ITGA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1043-1072 amino acids from isoform 2 of human ITGA6.
Usage
Immunohistochemistry: LS-B3626 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3626 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGA6/Integrin Alpha 6/CD49f
P23229 NM_000210 NP_000201.2

Validation

Anti-ITGA6 / CD49f antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ITGA6 / CD49f antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ITGA6 / CD49f antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-ITGA6 / CD49f antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ITGA6 / CD49f antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ITGA6 / CD49f antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/17/2019