Target
Mouse IL27
Synonyms
IL27 | IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
Specificity
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:1000 - 1:5000)
Usage
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kD in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee