The biotinylated polyclonal rabbit anti-mouse IL-1beta antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-1beta in combination with the unlabelled B122 (LS-C107128
) antibody for capture and recombinant mouse IL-1beta as the standard. A suitable range of concentrations of this antibody for ELISA detection is 4.0 - 6.0 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.