Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C744648-100 100 µl (85 mg/ml) $567 
IL-10 Antibody - Western Blot of rabbit anti-IL-10 antibody. Lane 1: Recombinant Human IL-10. Load: 50 ng per lane. Primary antibody: IL-10 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for IL-10.
IL-10 Antibody - Anti IL-10 whole antiserum was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n 109-401-312 diluted 1:200 in 3% BSA-TBS o/n 4oC. Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Biorad. Other detection systems will yield similar results.
IL-10 Antibody - Western Blot of rabbit anti-IL-10 antibody. Lane 1: Recombinant Human IL-10. Load: 50 ng per lane. Primary antibody: IL-10 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for IL-10.
IL-10 Antibody - Anti IL-10 whole antiserum was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n 109-401-312 diluted 1:200 in 3% BSA-TBS o/n 4oC. Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Biorad. Other detection systems will yield similar results.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human IL‑10 Antibody (WB) LS‑C744648

Polyclonal Rabbit anti‑Human IL‑10 Antibody (WB) LS‑C744648

Antibody:
IL-10 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C744648-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL-10 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL-10 antibody LS-C744648 is an unconjugated rabbit polyclonal antibody to human IL-10. Validated for ELISA and WB.
Target
Human IL-10
Synonyms
IL10 | CSIF | GVHDS | IL-10 | IL10A | Interleukin-10 | Interleukin 10 | TGIF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
The whole rabbit serum was prepared by repeated immunizations with human IL-10.
Specificity
The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.
Applications
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Usage
Applications should be user optimized.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-10
P22301 NM_000572 NP_000563.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/7/2024