Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B4913-0.1 0.1 mg (1 mg/ml) $440 
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse testis. Positive staining observed within the spermatocytes and no staining within the spermatids, spermatozoa, and septa.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse spleen. Positive staining observed within a subset of lymphocytes.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse small intestine. Positive cytoplasmic staining within absorptive epithelium of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IL-10 Antibody - Western blot of mouse IL-10 recombinant protein probed with Rat anti-Mouse Interleukin-10 antibody followed by F(ab')2 Rabbit anti-Rat IgG:HRP.
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse testis. Positive staining observed within the spermatocytes and no staining within the spermatids, spermatozoa, and septa.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse spleen. Positive staining observed within a subset of lymphocytes.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse small intestine. Positive cytoplasmic staining within absorptive epithelium of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IL-10 Antibody - Western blot of mouse IL-10 recombinant protein probed with Rat anti-Mouse Interleukin-10 antibody followed by F(ab')2 Rabbit anti-Rat IgG:HRP.
1 of 4
2 of 4
3 of 4
4 of 4

PathPlus™ Monoclonal Rat anti‑Mouse IL‑10 Antibody (clone JES5‑2A5, IHC, WB) LS‑B4913

PathPlus™ Monoclonal Rat anti‑Mouse IL‑10 Antibody (clone JES5‑2A5, IHC, WB) LS‑B4913

Note: This antibody replaces LS-C43440
Antibody:
IL-10 Rat anti-Mouse Monoclonal (JES5-2A5) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo, ELISA
Reactivity:
Mouse, Goat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B4913-0.1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IL-10 Rat anti-Mouse Monoclonal (JES5-2A5) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo, ELISA
Reactivity:
Mouse, Goat
Format:
Unconjugated, Unmodified

Specifications

Description
IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. In immunohistochemistry, it has plasma positivity and cytoplasmic staining in a few tissues. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804;
Target
Mouse IL-10
Synonyms
IL10 | CSIF | GVHDS | IL-10 | IL10A | Interleukin-10 | Interleukin 10 | TGIF
Host
Rat
Reactivity
Mouse, Goat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
JES5-2A5
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant mouse IL-10.
Specificity
Ârecognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells and a subset of stimulated T cells. IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response. Is reported to neutralize the activity of murine IL-10.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:100)
  • ELISA (1:200 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Membrane permeabilization is required for this application.
Presentation
PBS, 0.09% Sodium Azide
Storage
Upon receipt, aliquot and store at -20°C. May be stored at 4°C for up to 1 month. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-10
P22301 NM_000572 NP_000563.1

Validation

IL-10 Antibody - Anti-IL-10 antibody IHC of mouse testis. Positive staining observed within the spermatocytes and no staining within the spermatids, spermatozoa, and septa.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-IL-10 antibody IHC of mouse testis. Positive staining observed within the spermatocytes and no staining within the spermatids, spermatozoa, and septa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-IL-10 antibody IHC of mouse testis. Positive staining observed within the spermatocytes and no staining within the spermatids, spermatozoa, and septa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IL-10 Antibody - Anti-IL-10 antibody IHC of mouse spleen. Positive staining observed within a subset of lymphocytes.  Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-IL-10 antibody IHC of mouse spleen. Positive staining observed within a subset of lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-IL-10 antibody IHC of mouse spleen. Positive staining observed within a subset of lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IL-10 Antibody - Anti-IL-10 antibody IHC of mouse small intestine. Positive cytoplasmic staining within absorptive epithelium of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-IL-10 antibody IHC of mouse small intestine. Positive cytoplasmic staining within absorptive epithelium of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-IL-10 antibody IHC of mouse small intestine. Positive cytoplasmic staining within absorptive epithelium of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

PathPlus™ IL-10 Antibody (clone JES5-2A5) for IHC, WB/Western, Flow, ELISA LS-B4913 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Skin-Derived Mesenchymal Stem Cells Alleviate Atherosclerosis via Modulating Macrophage Function. Li Q, Sun W, Wang X, Zhang K, Xi W, Gao P. Stem cells translational medicine. 2015 November;4:1294-301. (Mouse)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021