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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
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IL-10

interleukin 10

IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Gene Name: interleukin 10
Family/Subfamily: Hormone , not assigned-Hormone
Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF
Target Sequences: NM_000572 NP_000563.1 P22301

Publications (2)

1
Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity. Brodeur ND, Spencer JV. Virus research. 2010 153:265-8. (WB; Human) [Full Text Article] [PubMed:20728483] [PMC:PMC3769974] Related Antibodies: LS-C47137.
2
Skin-Derived Mesenchymal Stem Cells Alleviate Atherosclerosis via Modulating Macrophage Function. Li Q, Sun W, Wang X, Zhang K, Xi W, Gao P. Stem cells translational medicine. 2015 November;4:1294-301. (Mouse) [Full Text Article] [PubMed:26400926] [PMC:PMC4622403] Related Antibodies: LS-B4913.

Viewing 1-25 of 176 items
IL-10 Antibody - Anti-IL-10 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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(1) Publication(s)
Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL-10
Mouse
ELISA, Flo, IHC, IHC-Fr, IHC-P, WB
Unconjugated
100µg   1mg/ml   $425.00
IL-10 Antibody - Anti-IL-10 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Rabbit Polyclonal (IgG) to Human IL-10
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $425.00
IL-10 Antibody - Direct ELISA of IL-10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Human IL-10
Human
ELISA, WB
Biotin Conjugated
25µg   $285.00; 50µg   $325.00
IL-10 Antibody - Western blot of human IL-10 recombinant protein probed with Rat anti-Human Interleukin-10 (RAT ANTI HUMAN INTERLEUKIN-10) followed by F(ab)2 Rabbit anti-Rat IgG:HRP (STAR21B).
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Rat Monoclonal [clone JES3-12G8] (IgG2a) to Human IL-10
Human
ELISA, Flo, IHC, IHC-P, WB
Unconjugated
0.5mg   1mg/ml   $455.00
Select
Mouse Monoclonal (IgG1) to Human IL-10
Human
ELISA, IP, WB
Unconjugated
500µg   $515.00; 1mg   $755.00
Select
Mouse Monoclonal [clone CC320] (IgG1) to Bovine IL-10
Bovine
ELISA, Flo
Unconjugated, Low Endotoxin
0.5mg   1mg/ml   $590.00
IL-10 Antibody
Select
(1) Publication(s)
Mouse Monoclonal [clone 3C12C12] (IgG1,k) to Human IL-10
Human
ELISA, WB
Unconjugated
100µg   1mg/ml   $275.00
IL-10 Antibody - Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Rabbit Polyclonal to Human IL-10
Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
IL-10 Antibody - Western Blot (non-reducing) of IL-10 antibody
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Rat Monoclonal [clone 15B9-1-2] (IgG2a) to Mouse IL-10
Mouse
ELISA, WB
Unconjugated
500µg   $255.00
Select
Mouse Monoclonal (IgG1,k) to Pig IL-10
Pig
ELISA
Unconjugated
500µg   $650.00
IL-10 Antibody - WB of IL10 antibody. Lane 1: Recombinant Rat IL10 Protein 10ng. Lane 2: Recombinant Rat IL10 Protein 5ng. Lane 3: Recombinant Rat IL10 Protein 2.5ng..
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Rabbit Polyclonal to Rat IL-10
Rat
ELISA, IHC, IHC-P, WB
Unconjugated
100µg   200µl   0.5mg/ml   $355.00
Select
Mouse Monoclonal [clone 945A5D11] (IgG1,k) to Human IL-10
Human
ELISA
Unconjugated
500µg   $605.00
Select
Mouse Monoclonal (IgG1) to Human IL-10
Human
ELISA
FITC Conjugated
100tst   $620.00
IL-10 Antibody - Western blot of IL-10 antibody.
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Rabbit Polyclonal to Dog IL-10
Dog
WB
Unconjugated
20µl   0.5mg/ml   $205.00; 100µl   0.5mg/ml   $265.00; 200µl   0.5mg/ml   $305.00; 1ml   0.5mg/ml   $545.00
IL-10 Antibody - Western Blot; Sample: Recombinant IL10, Rat.
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Mouse Monoclonal (IgG2a,k) to Rat IL-10
Rat
WB
Unconjugated
20µl   1mg/ml   $205.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $305.00; 1ml   1mg/ml   $545.00
IL-10 Antibody - Immunohistochemistry of paraffin-embedded mouse heart.
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Rabbit Polyclonal (IgG) to Human IL-10
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl   2.89mg/ml   $235.00; 100µl   $315.00; 200µl   $425.00
IL-10 Antibody - Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant proteinIL-10. The membrane blot was probed with antiALPP primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein IL-10 from E.coli cell (19kDa).
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Rabbit Polyclonal (IgG) to Human IL-10
Human
ELISA, WB
Unconjugated, Preservative Free
20µg   $195.00; 100µg   $265.00; 200µg   $295.00; 500µg   $415.00
Select
Mouse Monoclonal [clone CC320] (IgG1) to Bovine IL-10
Bovine
ELISA
Biotin Conjugated
0.25mg   $620.00
Select
Rat Monoclonal (IgG2a) to Human IL-10
Human
ELISA, Flo, IHC, IHC-P, WB
Unconjugated
500µg   $725.00
Select
Rabbit Polyclonal (IgG) to Human IL-10
Human
ICC, IHC, IHC-Fr, Neut, WB
Unconjugated
100µg   $1,020.00
Select
Rat Monoclonal [clone JES3-9D7] (IgG1,k) to Human IL-10
Human
Flo, SwELISA, WB
Unconjugated
0.5mg   0.5mg/ml   $365.00
Select
Rabbit Polyclonal to Human IL-10
Human
ELISA, Neut, WB
Unconjugated
50µg   $365.00
Select
Mouse Monoclonal (IgG1) to Human IL-10
Human
ELISA, WB
Unconjugated
20µg   $195.00; 100µg   $265.00; 200µg   $295.00; 500µg   $415.00
IL-10 Antibody - Western blot analysis of IL10 using anti-IL10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysate,Lane 2: rat testis tissue lysates,Lane 3: mouse testis tissue lysates,Lane 4: mouse SP20 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
Select
Rabbit Polyclonal (IgG) to Human IL-10
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg   $325.00
Select
Rat Monoclonal [clone JES5-16E3] (IgG2b,k) to Mouse IL-10
Mouse
ELISA
Unconjugated
500µg   $455.00

Viewing 1-25 of 176 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).