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IHCPlus™ TGM2 / Transglutaminase 2 Antibody Polyclonal Azide‑free IHC,IP,WB LS‑B2681

Catalog Number Size Price
LS-B2681-50 50 µl (1 mg/ml) $425 
Anti-TGM2 / Transglutaminase 2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

IHCPlus™ TGM2 / Transglutaminase 2 Antibody Polyclonal Azide‑free IHC,IP,WB LS‑B2681

Note: This antibody replaces LS-C39836, LS-C88595
Antibody:
Rabbit Polyclonal to Human TGM2 / Transglutaminase 2
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Azide-free
Other formats:
Price
Catalog Number
$425 
LS-B2681-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TGM2 / Transglutaminase 2
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Azide-free
Other formats:

Specifications

Description
Transglutaminase 2 antibody LS-B2681 is an unconjugated rabbit polyclonal antibody to Transglutaminase 2 (TGM2 ) from human, mouse and rat. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TGM2 / Transglutaminase 2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (5 - 10 µg/ml)
  • Immunoprecipitation (10 µg/tst)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.
Specificity
Recognizes Transglutaminase II. Species cross-reactivity: Human, mouse, rat.
Usage
Immunohistochemistry: LS-B2681 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2681 was determined to be 5 ug/ml. Immunoprecipitation (Native verified): 10 ug/mg protein lysate. Use Protein A. Western Blot: 5-10 ug/ml for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TGM2 / Transglutaminase 2
P21980 NM_004613 NP_004604.2

Validation

Anti-TGM2 / Transglutaminase 2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC Validated
Anti-TGM2 / Transglutaminase 2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-TGM2 / Transglutaminase 2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining of tumor and endothelial cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
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Anti-TGM2 / Transglutaminase 2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
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Anti-TGM2 / Transglutaminase 2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
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Requested From: United States
Date Requested: 9/18/2019