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TGM2 / Transglutaminase 2 Antibody (Lys444) LS‑C97227

Catalog Number Size Price
LS-C97227-400 400 µl $325 
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TGM2 immunoreactivity is localized to Cytoplasm significantly.
TGM2 Antibody (Center K444) western blot of HUVEC,K562 cell line lysates (35 ug/lane). The TGM2 antibody detected the TGM2 protein (arrow).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TGM2 immunoreactivity is localized to Cytoplasm significantly.
TGM2 Antibody (Center K444) western blot of HUVEC,K562 cell line lysates (35 ug/lane). The TGM2 antibody detected the TGM2 protein (arrow).
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TGM2 / Transglutaminase 2 Antibody (Lys444) LS‑C97227

Antibody:
Rabbit Polyclonal to Human TGM2 / Transglutaminase 2
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C97227-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TGM2 / Transglutaminase 2
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Transglutaminase 2 antibody LS-C97227 is an unconjugated rabbit polyclonal antibody to human Transglutaminase 2 (TGM2 ). Validated for IF, IHC and WB.
Target
Human TGM2 / Transglutaminase 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9294 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Lys444
Specificity
This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2).
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TGM2 / Transglutaminase 2
P21980 NM_004613 NP_004604.2

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Requested From: United States
Date Requested: 9/17/2019